IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of human skeletal muscle tissue stained with GSK-3beta, pAb at 10µg/ml.)
Rabbit GSK-3beta Polyclonal Antibody | anti-GSK-3beta antibody
GSK-3beta polyclonal antibody
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Protein A affinity purified.
Synonyms
GSK-3beta, Antibody; GSK-3beta polyclonal antibody; Glycogen synthase kinase-3beta; GSK-3beta, pAb; anti-GSK-3beta antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Specificity
Recognizes human, mouse, rat and bovine GSK-3beta. Detects a band of ~47kDa by Western blot.
Purity/Purification
Protein A affinity purified.
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Sequence Length
420
Applicable Applications for anti-GSK-3beta antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Immunogen
Synthetic peptide corresponding to a portion of rat GSK-3beta. The sequence is completely conserved in human.
Preparation and Storage
Long Term: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of human skeletal muscle tissue stained with GSK-3beta, pAb at 10µg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of human skeletal muscle tissue stained with GSK-3beta, pAb at 10µg/ml.)
WB (Western Blot)
(Western blot analysis of human HeLa (heat shocked) cell lysate, probed with GSK3-beta pAb)
WB (Western Blot)
(Western blot analysis of human HeLa (heat shocked) cell lysate, probed with GSK3-beta pAb)
Related Product Information for anti-GSK-3beta antibody
Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.
Product Categories/Family for anti-GSK-3beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
46,742 Da
NCBI Official Full Name
Glycogen synthase kinase-3 beta
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
Gsk3b
NCBI Protein Information
glycogen synthase kinase-3 beta
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
Gsk3b
UniProt Synonym Gene Names
GSK-3 beta; FA
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Product Notes
The GSK-3beta gsk3b (Catalog #AAA77018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSK-3beta polyclonal antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's GSK-3beta can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the GSK-3beta gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK-3beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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