GSK3beta recombinant protein

GSK3beta, Active

Cat. Num. AAA72987

• Applications: Western Blot
• Synonyms: GSK3beta; N/A; GSK3beta, Active; GSK3beta recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full Length
• Sequence Length: 7134

• Applicable Applications for GSK3beta recombinant protein: WB (Western Blot), Kinase Assay
• Species: Human
• Tag: GST tag
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Application Data

Application Data

Related Product Information for GSK3beta recombinant protein

Description: Recombinant full-length human GSK3beta was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.
Scientific Background: GSK3beta is a serine threonine protein kinase that was originally identified as the kinase that phosphorylate and inhibits glycogen synthase (1). GSK3beta is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin, modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3 beta into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies (2).

Product Categories/Family for GSK3beta recombinant protein

Enzymes; Active Kinases

References

1. Sutherland C. et al: Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling. Biochem J. 1993 Nov 15;296 (Pt 1):15-9. 2. Sperber BR, Leight S, Goedert M, Lee VM. Glycogen synthase kinase-3 beta phosphorylates tau protein at multiple sites in intact cells. Neurosci Lett. 1995 Sep 8;197 (2):149-53.

NCBI and Uniprot Product Information

• NCBI GI #: 225903415
• NCBI GeneID: 2932
• NCBI Accession #: NM_002093.3
• NCBI GenBank Nucleotide #: NM_002093
• Molecular Weight: ~73 kDa
• NCBI Official Full Name: Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta