ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using GSK-3? mouse mAb (dilution 1:200).)
Mouse anti-Human, Mouse GSK3 beta Monoclonal Antibody | anti-GSK3beta antibody
GSK3 beta Mouse mAb
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
GSK3 beta, Antibody; GSK3 beta Mouse mAb; Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B_HUMAN; GSK3beta isoform; Serine/threonine-protein kinase GSK3B.; anti-GSK3beta antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of GSK-3beta and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GSK3beta antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant GSK-3beta protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using GSK-3? mouse mAb (dilution 1:200).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using GSK-3? mouse mAb (dilution 1:200).)
WB (Western Blot)
(Western blot analysis of extracts from A549. Hela. 3T3 and Rat Brain cell lysates using GSK-3? mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
WB (Western Blot)
(Western blot analysis of extracts from A549. Hela. 3T3 and Rat Brain cell lysates using GSK-3? mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using GSK-3?.GSK-3? Rabbit mAb was used for the western blot analysis (1:1000 diluted).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using GSK-3?.GSK-3? Rabbit mAb was used for the western blot analysis (1:1000 diluted).)
Related Product Information for anti-GSK3beta antibody
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Product Categories/Family for anti-GSK3beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,744 Da
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 2
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B
NCBI Protein Information
glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; serine/threonine-protein kinase GSK3B
UniProt Protein Name
Glycogen synthase kinase-3 beta
UniProt Gene Name
GSK3B
UniProt Synonym Gene Names
GSK-3 beta
UniProt Entry Name
GSK3B_HUMAN
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Product Notes
The GSK3beta gsk3b (Catalog #AAA320326) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSK3 beta Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3 beta can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the GSK3beta gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3 beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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