GST A3-3 recombinant protein
GST A3-3, Recombinant Human
Gene Names
GSTA3; GTA3; GSTA3-3
Purity
95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Synonyms
GST A3-3; N/A; GST A3-3, Recombinant Human; Recombinant Human GST A3-3; GST A3-3 recombinant protein
Host
Human recombinant protein expressed in E Coli.
Specificity
Specific Activity: 61 U/mg using spectrophotometric determination of 1-chloro- 2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.
Purity/Purification
95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Concentration
1.0 mg/mL total protein using the Bradford protein assay with BSA as a standard. (varies by lot)
Sequence Length
222
Storage Buffer
1.0 mg/mL total protein using the Bradford protein assay with BSA as a standard.
Procedural Notes
This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity toward CDNB and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than CDNB.
Preparation and Storage
At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
~25 kDa
NCBI Official Full Name
Glutathione S-transferase A3
NCBI Official Synonym Full Names
glutathione S-transferase alpha 3
NCBI Official Symbol
GSTA3
NCBI Official Synonym Symbols
GTA3; GSTA3-3
NCBI Protein Information
glutathione S-transferase A3; GST class-alpha member 3; S-(hydroxyalkyl)glutathione lyase A3; glutathione S-alkyltransferase A3; glutathione S-aralkyltransferase A3; glutathione S-aryltransferase A3; glutathione S-transferase A3-3
UniProt Protein Name
Glutathione S-transferase A3
UniProt Gene Name
GSTA3
UniProt Entry Name
GSTA3_HUMAN
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Product Notes
The GST A3-3 gsta3 (Catalog #AAA71851) is a Recombinant Protein produced from Human recombinant protein expressed in E Coli. and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GST A3-3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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