SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
HAT1 recombinant protein
HAT1, 20-341aa, Human, His tag, E Coli
Gene Names
HAT1; KAT1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
HAT1; N/A; HAT1, 20-341aa, Human, His tag, E Coli; Histone acetyltransferase 1; KAT1; Histone acetyltransferase 1 HAT 1; Histidine aminotransferase 1; Histone acetyltransferase type B catalytic subunit; HAT1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT.
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MKKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD
Sequence Length
419
Applicable Applications for HAT1 recombinant protein
SDS-PAGE
Tag Information
His-tag
Domain
20-341aa
Preparation and Storage
Can be stored at 2-8 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for HAT1 recombinant protein
HAT1, also known as histone acetyltransferase 1, is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Recombinant HAT1 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HAT1 recombinant protein
References
Verreault A., et al. (1998) Curr Biol. 8 (2):96-108. ; Marmorstein R., et al. (2001) J Mol Biol. 311 (3):433-44. ;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40 kDa (343aa), confirmed by MALDI-TOF.
NCBI Official Full Name
histone acetyltransferase type B catalytic subunit
NCBI Official Synonym Full Names
histone acetyltransferase 1
NCBI Official Symbol
HAT1
NCBI Official Synonym Symbols
KAT1
NCBI Protein Information
histone acetyltransferase type B catalytic subunit
UniProt Protein Name
Histone acetyltransferase type B catalytic subunit
UniProt Gene Name
HAT1
UniProt Synonym Gene Names
KAT1
Similar Products
Product Notes
The HAT1 hat1 (Catalog #AAA48631) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag!!Domain||20-341aa. AAA Biotech's HAT1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HAT1 hat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MKKLAEYKCN TNTAIELKLV RFPEDLENDI RTFFPEYTHQ LFGDDETAFG YKGLKILLYY IAGSLSTMFR VEYASKVDEN FDCVEADDVE GKIRQIIPPG FCTNTNDFLS LLEKEVDFKP FGTLLHTYSV LSPTGGENFT FQIYKADMTC RGFREYHERL QTFLMWFIET ASFIDVDDER WHYFLVFEKY NKDGATLFAT VGYMTVYNYY VYPDKTRPRV SQMLILTPFQ GQGHGAQLLE TVHRYYTEFP TVLDITAEDP SKSYVKLRDF VLVKLCQDLP CFSREKLMQG FNEDMAIEAQ QKFKINKQHA RRVYEILRLL VTD. It is sometimes possible for the material contained within the vial of "HAT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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