Standard Curve (Sample)
Human Cytomegalovirus Glycoprotein B (HCMV gB) ELISA Kit | HCMVgB elisa kit
Human Cytomegalovirus Glycoprotein B (HCMV gB) ELISA Kit
Reactivity
Human
Synonyms
Cytomegalovirus Glycoprotein B (HCMV gB); N/A; Human Cytomegalovirus Glycoprotein B (HCMV gB) ELISA Kit; HCMVgB elisa kit
Reactivity
Human
Detection Method
Colorimetric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HCMVgB elisa kit
Background: The human cytomegalovirus (HCMV) is a widespread pathogen responsible for generally asymptomatic and persistent infections in healthy people. It may, however, cause severe disease in the absence of an effective immune response. HCMV is a member of the herpesviruses. The virion of HCMV consists of a 100-nm diameter icosahedral nucleocapsid containing a 230-kbp, double-stranded linear DNA genome surrounded by a proteinaceous layer defined as the tegument or matrix which, in turn, is enclosed by a lipid bilayer containing a large number of viral glycoproteins. The mature virion particle is 150-200 nm in diameter. Glycoprotein B (gB), an abundant glycoprotein on the virus envelope and the most highly conserved glycoprotein of the human herpesviruses, is required for virus entry and cell-to-cell spread of the virus. The HCMV gB ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the HCMV gB protein. The kit has detection sensitivity limit of 625 pg /mL HCMV gB. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and HCMV samples.
Principle of the Assay: An anti-HCMV gB monoclonal coating antibody is adsorbed onto a microtiter plate. HCMV gB protein present in the sample or standard binds to the antibody adsorbed on the plate; a FITC-conjugated anti-HCMV gB monoclonal antibody is added and binds to the HCMV gB protein captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse antiFITC antibody is added and binds to the FITC conjugated anti-HCMV gB monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and a substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HCMV gB protein present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from the provided HCMV gB protein standard and the sample HCMV gB protein concentration is then determined.
Principle of the Assay: An anti-HCMV gB monoclonal coating antibody is adsorbed onto a microtiter plate. HCMV gB protein present in the sample or standard binds to the antibody adsorbed on the plate; a FITC-conjugated anti-HCMV gB monoclonal antibody is added and binds to the HCMV gB protein captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse antiFITC antibody is added and binds to the FITC conjugated anti-HCMV gB monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and a substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HCMV gB protein present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from the provided HCMV gB protein standard and the sample HCMV gB protein concentration is then determined.
Product Categories/Family for HCMVgB elisa kit
Similar Products
Product Notes
The Human HCMVgB (Catalog #AAA44706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA44706 ELISA Kit recognizes Human HCMVgB. It is sometimes possible for the material contained within the vial of "Cytomegalovirus Glycoprotein B (HCMV gB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.