Application Data
(HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
HDAC5 recombinant protein
Recombinant HDAC5 protein
Gene Names
HDAC5; HD5; NY-CO-9
Purity
The purity of HDAC5 protein is >66% by SDS-PAGE.
Synonyms
HDAC5; N/A; Recombinant HDAC5 protein; HDAC-5; Histone Deacetylase 5; HD5; HDAC5 recombinant protein
Host
Baculovirus
Purity/Purification
The purity of HDAC5 protein is >66% by SDS-PAGE.
Form/Format
Recombinant full length HDAC5 protein is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, and 0.2mM TCEP.
Concentration
0.15ug/ul (varies by lot)
Source
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on Dry Ice.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data
(HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data
(HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data
(HDAC-GloTM Class IIa Assay for HDAC5 activity Assay was performed using HDAC-Glo™ Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC5 proteins and 1/20000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
SDS-PAGE
(Recombinant HDAC5 protein gel. HDAC5 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant HDAC5 protein gel. HDAC5 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for HDAC5 recombinant protein
Description: Recombinant human HDAC5 was expressed in a baculovirus expression system as the full-length protein (accession number NP_001015053.1) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 124 kDa. The purity of HDAC5 protein is >66% by SDS-PAGE.
Background: HDAC5 (Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modi cation that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue.In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins.
Background: HDAC5 (Histone Deacetylase 5) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modi cation that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue.In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).Like HDAC4, HDAC5 shuttles between the nucleus and cytoplasm. In muscle cells, this protein shuttles into the cytoplasm during myocyte differentiation. HDAC5 interacts with many different transcription factors including HDAC3, and may form multi-complex proteins.
Product Categories/Family for HDAC5 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone deacetylase 5 isoform 3
NCBI Official Synonym Full Names
histone deacetylase 5
NCBI Official Symbol
HDAC5
NCBI Official Synonym Symbols
HD5; NY-CO-9
NCBI Protein Information
histone deacetylase 5; antigen NY-CO-9
UniProt Protein Name
Histone deacetylase 5
UniProt Gene Name
HDAC5
UniProt Synonym Gene Names
KIAA0600; HD5
UniProt Entry Name
HDAC5_HUMAN
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Product Notes
The HDAC5 hdac5 (Catalog #AAA59739) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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