ELISA
(SARS COV2 S B.1.1.529 trimer ACE functional binding ELISA assay2019-ncov S B.1.1.529 trimer(coating at 0.1ug-well) binding with Human ACE2-Fc.The linear range was found to be 23 ng/ml)
SARS COV2 Spike protein S B.1.1.529 (omicron) Trimer Active Protein | SARSCOV2 active protein
SARS COV2 Spike protein S B.1.1.529 (omicron) Trimer Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
SARS COV2 Spike protein S B.1.1.529 (omicron) Trimer; N/A; SARS COV2 Spike protein S B.1.1.529 (omicron) Trimer Recombinant; NCOVS; SARSCOV2 active protein
Host
HEK cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant nCoV-2019 Spike S trimer protein was lyophilized from 0.2 um filtered PBS pH7.4.
Source
DNA sequence encoding the COVID-19 Spike S protein [accession# QIC53204.1] variant (B.1.1.529/Omicron) (A67V, del69-70, T95I, del142-144, Y145D, del211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) fused to a a T4 Folsom trimerzation domain and a C-terminal His AVI tag was expressed in HEK Cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
ELISA
(SARS COV2 S B.1.1.529 trimer ACE functional binding ELISA assay2019-ncov S B.1.1.529 trimer(coating at 0.1ug-well) binding with Human ACE2-Fc.The linear range was found to be 23 ng/ml)
ELISA
(SARS COV2 S B.1.1.529 trimer ACE functional binding ELISA assay2019-ncov S B.1.1.529 trimer(coating at 0.1ug-well) binding with Human ACE2-Fc.The linear range was found to be 23 ng/ml)
Tris-Bis-PAGE
(S B.1.1.529 trimer protein PAGE1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
Tris-Bis-PAGE
(S B.1.1.529 trimer protein PAGE1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
Product Categories/Family for SARSCOV2 active protein
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Product Notes
The SARSCOV2 (Catalog #AAA76382) is an Active Protein produced from HEK cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SARS COV2 Spike protein S B.1.1.529 (omicron) Trimer, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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