ErbB4/Her4 active protein

Recombinant human ErbB4/Her4 protein

Cat. Num. AAA48406

• Gene Names: ERBB4; HER4; ALS19; p180erbB4
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: ErbB4/Her4; N/A; Recombinant human ErbB4/Her4 protein; Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; 4ICD; E4ICD; s80HER4; ERBB4; HER4; receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; proto-oncogene-like protein c-ErbB-4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4; human epidermal growth factor receptor 4; ALS19; ErbB4/Her4 active protein

• Host: HEK293
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 26-649aa
• Sequence: QSVCAGTENK LSSLSDLEQQ YRALRKYYEN CEVVMGNLEI TSIEHNRDLS FLRSVREVTG YVLVALNQFR YLPLENLRII RGTKLYEDRY ALAIFLNYRK DGNFGLQELG LKNLTEILNG GVYVDQNKFL CYADTIHWQD IVRNPWPSNL TLVSTNGSSG CGRCHKSCTG RCWGPTENHC QTLTRTVCAE QCDGRCYGPY VSDCCHRECA GGCSGPKDTD CFACMNFNDS GACVTQCPQT FVYNPTTFQL EHNFNAKYTY GAFCVKKCPH NFVVDSSSCV RACPSSKMEV EENGIKMCKP CTDICPKACD GIGTGSLMSA QTVDSSNIDK FINCTKINGN LIFLVTGIHG DPYNAIEAID PEKLNVFRTV REITGFLNIQ SWPPNMTDFS VFSNLVTIGG RVLYSGLSLL ILKQQGITSL QFQSLKEISA GNIYITDNSN LCYYHTINWT TLFSTINQRI VIRDNRKAEN CTAEGMVCNH LCSSDGCWGP GPDQCLSCRR FSRGRICIES CNLYDGEFRE FENGSICVEC DPQCEKMEDG LLTCHGPGPD NCTKCSHFKD GPNCVEKCPD GLQGANSFIF KYADPDRECH PCHPNCTQGC NGPTSHDCIY YPWTGHSTLP QHAR

• Applicable Applications for ErbB4/Her4 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Gln26
• Bioactivity: Measured by its binding ability in a functional ELISA with Human NRG1/HRG1. The ED50 range <= 0.2ug/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human NRG1/HRG1 (CAT# ATGP3990) is coated at 10 ug/ml (100 ul/well) can bind Human ERBB4. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for ErbB4/Her4 active protein

ErbB4, also known as Her4, is a receptor tyrosine kinase that is a member of the epidermal growth factor receptor family. ErbB family members serve as receptors for the EGF family of growth factors. It is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. It is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer. Single-nucleotide polymorphisms in ERBB4 have also been found in a study of patients with familial amyotrophic lateral sclerosis type 19. Recombinant human ErbB4, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Categories/Family for ErbB4/Her4 active protein

Cancer; Signal Transduction; Proteins

References

Silberberg G., et al, (2006) American Journal of Medical Genetics Part B. 141B: 142-148. C Rio. et al, (2000) J Biol Chem. 275:10379-10387. Zimonjic DB., et al, (1995) Oncogene. 10:1235-1237.

NCBI and Uniprot Product Information

• NCBI GI #: 4885215
• NCBI GeneID: 2066
• NCBI Accession #: NP_005226.1
• NCBI GenBank Nucleotide #: NM_005235.2
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 143,968 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 4
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; ALS19; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; human epidermal growth factor receptor 4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblast
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN