Bioactivity
(Human IL-4 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
IL-4 active protein
Recombinant human IL-4 protein
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
IL-4; N/A; Recombinant human IL-4 protein; Interleukin-4 isoform 1; B cell growth factor 1; B-cell stimulatory factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1;BSF-1; interleukin 4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra; IL-4 active protein
Host
HEK293
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
25-153aa
Sequence
Applicable Applications for IL-4 active protein
Bioactivity, SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Mouse
Tag
His-Tag
Predicted N-term
Asp
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range <= 0.5ng/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Human IL-4 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
Bioactivity
(Human IL-4 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for IL-4 active protein
IL-4 is a multi-functional cytokine that plays a critical role in the regulation of immune responses. It induces differentiation of naive helper T cells to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. IL-4 is produced primarily by mast cells, Th2 cells, eosinophils and basophils. It is closely related and has functions similar to interleukin 13. This protein has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 plays a dominant role in the development of allergic inflammation and asthma. Also, it is crucial in the development of allergic airway inflammation originates. Recombinant human IL-4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Categories/Family for IL-4 active protein
References
Bhattarai P., et al, (2016) Cell Reports. 17: 941-948. Cheng Ho., et al, (2016) Adv Exp Med Biol. 941:31-77. Marsha Wills-Karp., et al, (2008) Sci Signal. 1:pe55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.2kDa (150aa), confirmed by MALDI-TOF.
NCBI Official Full Name
interleukin-4 isoform 1
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
IL4
NCBI Official Synonym Symbols
BSF1; IL-4; BCGF1; BSF-1; BCGF-1
NCBI Protein Information
interleukin-4
UniProt Protein Name
Interleukin-4
UniProt Gene Name
IL4
UniProt Synonym Gene Names
IL-4; BSF-1
UniProt Entry Name
IL4_HUMAN
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Product Notes
The IL-4 il4 (Catalog #AAA48382) is an Active Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 25-153aa. AAA Biotech's IL-4 can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the IL-4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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