VEGFR1/Flt-1 active protein

Recombinant mouse VEGFR1/Flt-1 protein

Cat. Num. AAA49007

• Gene Names: Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: VEGFR1/Flt-1; N/A; Recombinant mouse VEGFR1/Flt-1 protein; VEGFR1; VEGF R1; VEGFR-1; FLT-1; FLT1; FLT; sFlt1; Emrk2; FRT; Vascular endothelial growth factor receptor 1 isoform 1; Embryonic receptor kinase 2; Fms-like tyrosine kinase 1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular permeability factor receptor; fms-related tyrosine kinase 1; VEGFR1/Flt-1 active protein

• Host: HEK293
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 23-759aa
• Sequence: Tyr 23; YGSGSKLKVP ELSLKGTQHV MQAGQTLFLK CRGEAAHSWS LPTTVSQEDK RLSITPPSAC GRDNRQFCST LTLDTAQANH TGLYTCRYLP TSTSKKKKAE SSIYIFVSDA GSPFIEMHTD IPKLVHMTEG RQLIIPCRVT SPNVTVTLKK FPFDTLTPDG QRITWDSRRG FIIANATYKE IGLLNCEATV NGHLYQTNYL THRQTNTILD VQIRPPSPVR LLHGQTLVLN CTATTELNTR VQMSWNYPGK ATKRASIRQR IDRSHSHNNV FHSVLKINNV ESRDKGLYTC RVKSGSSFQS FNTSVHVYEK GFISVKHRKQ PVQETTAGRR SYRLSMKVKA FPSPEIVWLK DGSPATLKSA RYLVHGYSLI IKDVTTEDAG DYTILLGIKQ SRLFKNLTAT LIVNVKPQIY EKSVSSLPSP PLYPLGSRQV LTCTVYGIPR PTITWLWHPC HHNHSKERYD FCTENEESFI LDPSSNLGNR IESISQRMTV IEGTNKTVST LVVADSQTPG IYSCRAFNKI GTVERNIKFY VTDVPNGFHV SLEKMPAEGE DLKLSCVVNK FLYRDITWIL LRTVNNRTMH HSISKQKMAT TQDYSITLNL VIKNVSLEDS GTYACRARNI YTGEDILRKT EVLVRDSEAP HLLQNLSDYE VSISGSTTLD CQARGVPAPQ ITWFKNNHKI QQEPGIILGP GNSTLFIERV TEEDEGVYRC RATNQKGAVE SAAYLTVQGT SDKSNLE

• Applicable Applications for VEGFR1/Flt-1 active protein: Bioactivity, SDS-PAGE
• Species: Mouse
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Tag: hIgG-Tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Related Product Information for VEGFR1/Flt-1 active protein

VEGFR1 (Vascular Endothelial Growth Factor Receptor 1), also known as FLT-1 (Fms-like Tyrosine Kinase 1), belongs to the class III subfamily of receptor tyrosine kinases. While VEGFR1, VEGFR2, and VEGFR3 are mainly expressed on endothelial cells, VEGFR1 is uniquely expressed on macrophages and primarily plays inhibitory roles. Inhibitors of VEGFR are used in cancer treatment. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB, and PGF, playing essential roles in the development of embryonic vasculature, regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 binds VEGF with higher affinity than VEGFR2 but exhibits weaker kinase activity. The VEGF-kinase ligand/receptor signaling system is crucial for vascular development and the regulation of vascular permeability. Recombinant mouse VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for VEGFR1/Flt-1 active protein

Immunology, Enzymes & Proteases, Angiogenesis, Cardiovascular; Proteins

References

Peters, K.G. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8915-8919.
Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.
Herley M.T., et al., (1999), Biochem. Biophys. Res. Commun. 262:731-738.
Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.

NCBI and Uniprot Product Information

• NCBI GI #: 34328180
• NCBI GeneID: 14254
• NCBI Accession #: NP_034358.2
• NCBI GenBank Nucleotide #: NM_010228.3
• UniProt Accession #: P35969; O55094; Q61517
• Molecular Weight: 149,876 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 1
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• NCBI Protein Information: vascular endothelial growth factor receptor 1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• UniProt Gene Name: Flt1
• UniProt Synonym Gene Names: Emrk2; Flt; Vegfr1; VEGFR-1; FLT-1
• UniProt Entry Name: VGFR1_MOUSE