Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.)
ACVR2B active protein
ACVR2B Protein, Mouse, Recombinant (His & hFc Tag)
Gene Names
Acvr2b; ActRIIB
Purity
>97% as determined by SDS-PAGE
Synonyms
ACVR2B; N/A; ACVR2B Protein, Mouse, Recombinant (His & hFc Tag); Mouse ACVR2B Protein (His & Fc Tag); 4930516B21Rik Protein, Mouse; ActRIIB Protein, Mouse; activin A receptor, type IIB; ACVR2B active protein
Host
HEK293 Cells
Purity/Purification
>97% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Thr134
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Ser l9
Tag
C-human IgG1-Fc & His
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Thr 134) of mouse ACVR2B (NP_031423.1) precursor was fused with the Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Measured by its ability to bind biotinylated Human INHBA-his in functional ELISA.
2. Measured by its ability to bind biotinylated mouse INHBA-his in functional ELISA.
3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50ng/mL in the presence of 10ng/mL recombinant Activin A.
2. Measured by its ability to bind biotinylated mouse INHBA-his in functional ELISA.
3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50ng/mL in the presence of 10ng/mL recombinant Activin A.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.)
Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.)
SDS-PAGE
SDS-PAGE
Related Product Information for ACVR2B active protein
Background: ACVR2A and ACVR2B are two activin type II receptors. ACVR2B is integral to the activin and myostatin signaling pathway. Ligands such as activin and myostatin bind to ACVR2A and ACVR2B. Myostatin, a negative regulator of skeletal muscle growth, is regarded as a potential therapeutic target and binds to ACVR2B effectively, and to a lesser extent, to ACVR2A. The structure of human ACVR2B kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. Haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength. Defects in ACVR2B are a cause of left-right axis malformations.
References
Kosaki R, et al. (1999) Left-right axis malformations associated with mutations in ACVR2B, the gene for human activin receptor type IIB. Am J Med Genet. 82(1):70-6. Dupont S, et al. (2001) No evidence for linkage or for diabetes-associated mutations in the activin type 2B receptor gene (ACVR2B) in French patients with mature-onset diabetes of the young or type 2 diabetes. Diabetes 50(5):1219-21. Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18. Walsh S, et al. (2007) Activin-type II receptor B (ACVR2B) and follistatin haplotype associations with muscle mass and strength in humans. J Appl Physiol. 102(6):2142-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,542 Da
NCBI Official Full Name
activin receptor type-2B
NCBI Official Synonym Full Names
activin receptor IIB
NCBI Official Symbol
Acvr2b
NCBI Official Synonym Symbols
ActRIIB
NCBI Protein Information
activin receptor type-2B; ACTR-IIB; activin receptor type IIB
UniProt Protein Name
Activin receptor type-2B
UniProt Gene Name
Acvr2b
UniProt Synonym Gene Names
ACTR-IIB
UniProt Entry Name
AVR2B_MOUSE
Similar Products
Product Notes
The ACVR2B acvr2b (Catalog #AAA258136) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Thr13 4. It is sometimes possible for the material contained within the vial of "ACVR2B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.