BAFFR/TNFRSF13C Active Protein | TNFRSF13C active protein

BAFFR/TNFRSF13C Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated

Cat. Num. AAA258045

• Gene Names: TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BAFFR/TNFRSF13C; N/A; BAFFR/TNFRSF13C Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated; Human BAFFR/TNFRSF13C (Fc & AVI Tag), Biotinylated; BAFF-R Protein, Human; BAFFR Protein, Human; BROMIX Protein, Human; CD268 Protein, Human; CVID4 Protein, Human; prolixin Protein, Human; tumor necrosis factor receptor superfamily, member 13C; TNFRSF13C active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Arg2-Ala71

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ser
• Tag: C-human IgG1-Fc & AVI
• Protein Construction: A DNA sequence encoding the human TNFRSF13C (NP_443177.1)(Arg2-Ala71) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized Human BAFF/BLyS hFc at 2ug/mL (100uL/well) can bind Human BAFFR/TNFRSF13C hFc & AVI, Biotinylated. The EC50 of Human BAFFR/TNFRSF13C hFc & AVI, Biotinylated is 40-200ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Immobilized Human BAFF/BLyS hFc at 2 ug/mL (100 uL/well) can bind Human BAFFR/TNFRSF13C hFc & AVI, Biotinylated, the EC50 of Human BAFFR/TNFRSF13C hFc & AVI, Biotinylated is 40-200 ng/mL.)

SDS-PAGE

Related Product Information for TNFRSF13C active protein

Background: Tumor necrosis factor receptor superfamily, member 13C (TNFRSF13C) also known as B-cell-activating factor receptor (BAFFR) and CD268 antigen, is a member of the tumor necrosis factor receptor superfamily. A tumor necrosis factor receptor (TNFR), or death receptor, is a trimeric cytokine receptor that binds tumor necrosis factors (TNF). The receptor cooperates with an adaptor protein which is important in determining the outcome of the response. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors or tumour necrosis factor (TNFR), on their surface. Tumour necrosis factors (TNFR) detect the presence of extracellular death signals and, in response, they rapidly ignite the cell's intrinsic apoptosis machinery. It has been proposed that abnormally high levels of BAFFR/TNFRSF13C (CD268) may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells.

References

Ashkenazi A, et al. (1998) Death receptors: signaling and modulation. Science. 281(5381): 1305-8.Losi CG, et al. (2005) Mutational analysis of human BAFF receptor TNFRSF13C (BAFF-R) in patients with common variable immunodeficiency. J Clin Immunol. 25(5): 496-502.Hentges KE, et al. (2002) Tnfrsf13c (Baffr) is mis-expressed in tumors with murine leukemia virus insertions at Lvis22. Genomics. 80(2): 204-12.

NCBI and Uniprot Product Information

• NCBI GI #: 16445027
• NCBI GeneID: 115650
• NCBI Accession #: NP_443177.1
• NCBI GenBank Nucleotide #: NM_052945.3
• UniProt Accession #: Q96RJ3
• Molecular Weight: 18,935 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 13C
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 13C
• NCBI Official Symbol: TNFRSF13C
• NCBI Official Synonym Symbols: BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 13C; B cell-activating factor receptor; B-cell-activating factor receptor; BAFF receptor; BLyS receptor 3
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 13C
• UniProt Gene Name: TNFRSF13C
• UniProt Synonym Gene Names: BAFFR; BR3; BAFF-R
• UniProt Entry Name: TR13C_HUMAN