Standard Curve (Sample)
Human TNFRSF13C/BAFFR ELISA Kit | TNFRSF13C elisa kit
Human TNFRSF13C/BAFFR PicoKine ELISA Kit
Gene Names
TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
Reactivity
Human
Synonyms
TNFRSF13C/BAFFR; N/A; Human TNFRSF13C/BAFFR PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 13C; tumor necrosis factor receptor superfamily, member 13C; B Cell Activating Factor Receptor; B-cell-activating factor receptor; BAFF R; BAFF receptor; BAFF-R; BAFFR; BLyS receptor 3; BlySR3; BR 3; BR3; BROMIX; CD 268; CD268; CD268 antigen; CVID4; MGC138235; OTTHUMP00000028746; Prolixin; TNFRSF 13C; TNFRSF13C; TR13C_HUMAN; Tumor Necrosis Factor Receptor Subunit Member 13C; TNFRSF13C elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TNFRSF13C
Sequence Length
184
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
78 pg/ml - 5,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNFRSF13C elisa kit
Background: Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. Bcell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Principle of the Assay: The Picokine™ Human TNFRSF13C Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFRSF13C with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF13C. The detection antibody is a biotinylated antibody specific for TNFRSF13C. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF13C with immunogen: Expression system for standard: NS0; Immunogen sequence: S7-A71. The kit is analytically validated with ready-to-use reagents. To measure Human TNFRSF13C, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFRSF13C in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF13C in the sample.
Principle of the Assay: The Picokine™ Human TNFRSF13C Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TNFRSF13C with a 96-well strip plate that is pre-coated with antibody specific for TNFRSF13C. The detection antibody is a biotinylated antibody specific for TNFRSF13C. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human TNFRSF13C with immunogen: Expression system for standard: NS0; Immunogen sequence: S7-A71. The kit is analytically validated with ready-to-use reagents. To measure Human TNFRSF13C, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TNFRSF13C in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TNFRSF13C in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
18,935 Da
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 13C
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 13C
NCBI Official Symbol
TNFRSF13C
NCBI Official Synonym Symbols
BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
NCBI Protein Information
tumor necrosis factor receptor superfamily member 13C; BAFF receptor; BLyS receptor 3; B cell-activating factor receptor; B-cell-activating factor receptor
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 13C
UniProt Gene Name
TNFRSF13C
UniProt Synonym Gene Names
BAFFR; BR3; BAFF-R
UniProt Entry Name
TR13C_HUMAN
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Product Notes
The Human TNFRSF13C tnfrsf13c (Catalog #AAA11616) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11616 ELISA Kit recognizes Human TNFRSF13C. It is sometimes possible for the material contained within the vial of "TNFRSF13C/BAFFR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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