CD16a / FCGR3A active protein

Recombinant Human CD16a / FCGR3A Protein (176 Val, His & AVI tag)

Cat. Num. AAA173505

• Gene Names: FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Synonyms: CD16a / FCGR3A; N/A; Recombinant Human CD16a / FCGR3A Protein (176 Val, His & AVI tag); Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3;CD16;CD16A; CD16a / FCGR3A active protein

• Host: HEK293 Cells
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5%Trehalose, 5% Mannitol, 0.01% Tween 80.
• Sequence: Met 1-Gln 208

• Species: Human
• Tag: C-His & AVI
• Bio Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD16a-AVI-His (V158) at 10 ug/ml (100 ul/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.08-0.18 ug/ml.
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (400uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. This product is provided as lyophilized powder which is shipped with blue ice.

SDS-PAGE

Related Product Information for CD16a / FCGR3A active protein

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

NCBI and Uniprot Product Information

• NCBI GI #: 17389688
• NCBI GeneID: 2214
• Molecular Weight: Mol_Mass: 25.6 kDa; AP_Mol_Mass: 46 kDa
• NCBI Official Full Name: FCGR3A protein
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor IIIa
• NCBI Official Symbol: FCGR3A
• NCBI Official Synonym Symbols: CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• UniProt Gene Name: FCGR3A
• UniProt Synonym Gene Names: CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa