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Application Data
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SPR DataBiotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14?M as determined in SPR assay (Biacore T200).)
Fc gamma RIIIA/CD16a (F176) Recombinant Protein | CD16a recombinant protein
Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Recombinant
Gene Names
FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Fc gamma RIIIA/CD16a (F176); N/A; Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Recombinant; CD16a recombinant protein
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human Fc gamma RIIIA/CD16a (F176) was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human Fc gamma RIIIA/CD16a (F176) including a C-His-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
IgG-binding-protein, Receptor
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
Application Data
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SPR DataBiotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14?M as determined in SPR assay (Biacore T200).)
Application Data
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SPR DataBiotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14?M as determined in SPR assay (Biacore T200).)
ELISA
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein ELISA DataImmobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA.)
ELISA
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein ELISA DataImmobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA.)
Application Data
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SEC-HPLCThe purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.)
Application Data
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SEC-HPLCThe purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Tris-Bis PAGEBiotinylated Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Tris-Bis-PAGE
(Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Tris-Bis PAGEBiotinylated Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Product Categories/Family for CD16a recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
254
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor III-A
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
NCBI Official Symbol
FCGR3A
NCBI Official Synonym Symbols
CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor III-A; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor III-A
UniProt Gene Name
FCGR3A
UniProt Synonym Gene Names
CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa
UniProt Entry Name
FCG3A_HUMAN
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Product Notes
The CD16a fcgr3a (Catalog #AAA76157) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fc gamma RIIIA/CD16a (F176), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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