CD55/DAF Active Protein | CD55 active protein

CD55/DAF Protein, Human, Recombinant (His Tag)

Cat. Num. AAA27760

• Gene Names: CD55; CR; TC; DAF; CROM
• Purity: > 95% as determined by SDS-PAGE; > 90% as determined by SEC-HPLC
• Synonyms: CD55/DAF; N/A; CD55/DAF Protein, Human, Recombinant (His Tag); Human CD55/DAF Protein (His Tag); CR Protein, Human; CROM Protein, Human; DAF Protein, Human; TC Protein, Human; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD55 active protein

• Host: HEK293 Cells
• Purity/Purification: > 95% as determined by SDS-PAGE; > 90% as determined by SEC-HPLC
• Form/Format: Lyophilized from sterile PBS, pH7.4, 5% trehalose , 5% mannitol

• Source: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 35
• Tag: His-Tag
• Protein Construction: A DNA sequence encoding the human CD55 precursor (NP_000565.1)(Met 1-Ser 353) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: It is receommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
• Bio-Activity: Immobilized Human CD55 His (AAA27760) at 2ug/ml (100uL/well) can bind Human CD97 hFc. The EC₅₀ of Human CD97 hFc is 15-65ng/mL.
• Preparation and Storage: Samples are stable for twelve months from date of receipt at -20°C to -80°C. Store under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for CD55 active protein

Background: CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.

Product Categories/Family for CD55 active protein

Complement Activation Pathways

References

Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.

NCBI and Uniprot Product Information

• NCBI GI #: 10835143
• NCBI GeneID: 1604
• NCBI Accession #: NP_000565.1
• NCBI GenBank Nucleotide #: NM_000574.3
• UniProt Accession #: P08174; P09679; P78361; B1AP14; D3DT83; D3DT84
• Molecular Weight: The secreted recombinant human CD55 consists of 330 amino acids and has a predicted molecular mass of 36.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD55 is approximately 55-60 kDa due to glycosylation.
• NCBI Official Full Name: complement decay-accelerating factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• NCBI Official Symbol: CD55
• NCBI Official Synonym Symbols: CR; TC; DAF; CROM
• NCBI Protein Information: complement decay-accelerating factor; CD55 antigen
• UniProt Protein Name: Complement decay-accelerating factor
• UniProt Gene Name: CD55
• UniProt Synonym Gene Names: CR; DAF
• UniProt Entry Name: DAF_HUMAN