DKK1 active protein

DKK1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA257836

• Gene Names: DKK1; SK; DKK-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DKK1; N/A; DKK1 Protein, Human, Recombinant (hFc Tag); Human DKK-1 Protein (Fc Tag); DKK-1 Protein, Human; SK Protein, Human; dickkopf WNT signaling pathway inhibitor 1; DKK1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met2-His266

• Species: Human
• Activity: Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07ug/mL in the presence of 10ng/mL of mouse Wnt3a.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 32
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human DKK1 (NP_036374.1)(Met2-His266) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07ug/mL in the presence of 10 ng/mL of mouse Wnt3a.)

SDS-PAGE

Related Product Information for DKK1 active protein

Background: Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Product Categories/Family for DKK1 active protein

Canonical Wnt Pathway

References

Horwitz EM. (2004) Dkk-1-mediated expansion of adult stem cells. Trends Biotechnol. 22(8): 386-8.Jiang T, et al. (2009) Clinical significance of serum DKK-1 in patients with gynecological cancer. Int J Gynecol Cancer. 19(7): 1177-81.

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374.1
• NCBI GenBank Nucleotide #: NM_012242.2
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 28,672 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1; hDkk-1; dickkopf-1 like; dickkopf 1 homolog; dickkopf-like protein 1; dickkopf related protein-1
• UniProt Protein Name: Dickkopf-related protein 1
• UniProt Gene Name: DKK1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; hDkk-1
• UniProt Entry Name: DKK1_HUMAN