Application Data
(Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-0.8 ng/mL.)
Interferon alpha 2/IFNA2 Active Protein | IFNA2 active protein
Interferon alpha 2/IFNA2 Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Ifna2; Ifa2
Purity
>88% as determined by SDS-PAGE
Synonyms
Interferon alpha 2/IFNA2; N/A; Interferon alpha 2/IFNA2 Protein, Mouse, Recombinant (hFc Tag); Mouse IFNA2/Interferon alpha 2 Protein (Fc Tag); Ifa2 Protein, Mouse; interferon, alpha 2; IFNA2 active protein
Host
HEK293 Cells
Purity/Purification
>88% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Cys24-Glu190
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Glu
Tag
N-human IgG1-Fc
Protein Construction
A DNA sequence encoding the mouse IFNA2 (P01573)(Cys 24-Glu 190) was fused with the Fc region of human IgG1 at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-0.8ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNAR1-His at 10ug/ml (100uL/well) can bind mouse Fc-IFNA2. The EC50 of mouse Fc-IFNA2 is 0.8-1.9ug/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNAR1-His at 10ug/ml (100uL/well) can bind mouse Fc-IFNA2. The EC50 of mouse Fc-IFNA2 is 0.8-1.9ug/ml.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-0.8 ng/mL.)
Application Data
(Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.1-0.8 ng/mL.)
SDS-PAGE
SDS-PAGE
Related Product Information for IFNA2 active protein
Background: IFNA2 (Interferon Alpha 2) is a Protein Coding gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-alpha genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNalpha-2 are designated alpha-2a, alpha-2b, and alpha-2c. IFNalpha-2b is being the predominant allele while IFNalpha-2a is less predominant and IFNalpha-2c only a minor allelic variant.
References
Wang, et al. (2004) Fever of recombinant human interferon-alpha is mediated by opioid domain interaction with opioid receptor inducing prostaglandin E2. J Neuroimmunol. 156(1-2): 107-12.Groopman JE, et al. (1984) Recombinant alpha-2 interferon therapy for Kaposi's sarcoma associated with the acquired immunodeficiency syndrome. Ann Intern Med. 100(5): 671-6.Krueger JM, et al. (1987) Interferon alpha-2 enhances slow-wave sleep in rabbits. Int J Immunopharmacol. 9(1): 23-30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,922 Da
NCBI Official Full Name
interferon alpha-2
NCBI Official Synonym Full Names
interferon alpha 2
NCBI Official Symbol
Ifna2
NCBI Official Synonym Symbols
Ifa2
NCBI Protein Information
interferon alpha-2
UniProt Protein Name
Interferon alpha-2
UniProt Gene Name
Ifna2
UniProt Synonym Gene Names
IFN-alpha-2
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Product Notes
The IFNA2 ifna2 (Catalog #AAA258171) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Cys24-Glu1 90. It is sometimes possible for the material contained within the vial of "Interferon alpha 2/IFNA2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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