Carbonic Anhydrase IV Active Protein | CA4 active protein

Carbonic Anhydrase IV Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA258156

• Gene Names: Car4; Ca4; AW456718
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase IV; N/A; Carbonic Anhydrase IV Protein, Mouse, Recombinant (His Tag); Mouse Carbonic Anhydrase IV/Car4 Protein (His Tag); AW456718 Protein, Mouse; Ca4 Protein, Mouse; Car4 Protein, Mouse; RP23-167D6.1 Protein, Mouse; carbonic anhydrase IV; CA4 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser277

• Species: Mouse
• Activity: Measured by its esterase activity. The specific activity is >10pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed,with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for CA4 active protein

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO₂ hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3^- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

References

Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20.Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.

NCBI and Uniprot Product Information

• NCBI GI #: 6671678
• NCBI GeneID: 12351
• NCBI Accession #: NP_031633.1
• NCBI GenBank Nucleotide #: NM_007607.2
• UniProt Accession #: Q64444
• Molecular Weight: Predicted Molecular Mass: 31.08 kDa; The secreted recombinant mouse Car4 consists of 271 amino acids and has a calculated molecular mass of 31 kDa as estimated in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: carbonic anhydrase 4
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: Car4
• NCBI Official Synonym Symbols: Ca4; AW456718
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• UniProt Gene Name: Ca4
• UniProt Synonym Gene Names: Car4; CA-IV