Rabbit anti-Human Carbonic Anhydrase IV Monoclonal Antibody | anti-CA4 antibody

Recombinant Anti-Carbonic Anhydrase IV Antibody, Rabbit Monoclonal

Cat. Num. AAA254951

• Gene Names: CA4; CAIV; Car4; RP17
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Protein A
• Synonyms: Carbonic Anhydrase IV, Antibody; Recombinant Anti-Carbonic Anhydrase IV Antibody, Rabbit Monoclonal; Carbonic Anhydrase IV/CA4 Antibody, Rabbit MAb; carbonic anhydrase IV; Anti-CAIV Antibody; Anti-Car4 Antibody; Anti-RP17 Antibody; anti-CA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 039
• Specificity: Human Carbonic Anhydrase IV/CA4.; No cross-reactivity in ELISA with Human CA2, Human CA3, Human CA5A, Human CA5B, Human CA8, Human CA9, Human CA10, Human CA12, Human CA13, Human CA14
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CA4 antibody: IHC (Immunohistochemistry), ELISA
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Carbonic Anhydrase IV/CA4 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IV/CA4 (rh Carbonic Anhydrase IV/CA4).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Immunochemical staining of human CA4 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to renal tubules.)

IHC (Immunohistochemistry)

(Immunochemical staining of human CA4 in human lung with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to pulmonary capillaries.)

Related Product Information for anti-CA4 antibody

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO_{2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.}

References

Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.

NCBI and Uniprot Product Information

• NCBI GI #: 4502519
• NCBI GeneID: 762
• NCBI Accession #: NP_000708.1
• NCBI GenBank Nucleotide #: NM_000717.4
• UniProt Accession #: P22748; Q6FHI7; B4DQA4
• Molecular Weight: 11,599 Da
• NCBI Official Full Name: carbonic anhydrase 4 preproprotein
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: CA4
• NCBI Official Synonym Symbols: CAIV; Car4; RP17
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• UniProt Gene Name: CA4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_HUMAN