Carboxypeptidase B1 Active Protein | CPB1 active protein

Carboxypeptidase B1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA27761

• Gene Names: CPB1; CPB; PASP; PCPB
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Carboxypeptidase B1; N/A; Carboxypeptidase B1 Protein, Human, Recombinant (His Tag); Human Carboxypeptidase B1/CPB1 Protein (His Tag); CPB Protein, Human; PASP Protein, Human; PCPB Protein, Human; carboxypeptidase B1 (tissue); CPB1 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH6.5, 5% trehalose, 5% mannitol and 0.01% Tween80
• Sequence: Met1-Tyr417

• Source: Human
• Activity: Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254nM. The specific activity is >1000pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 16
• Tag: His-Tag
• Protein Construction: A DNA sequence encoding the human CPB1 (NP_001862.2)(Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: It is recommmended that sterile water (200ul)be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for CPB1 active protein

Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

References

Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581. Pezzilli, R. et al., 1994, Digestion. 55: 73-77. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.

NCBI and Uniprot Product Information

• NCBI GI #: 54607080
• NCBI GeneID: 1360
• NCBI Accession #: NP_001862.2
• NCBI GenBank Nucleotide #: NM_001871.2
• UniProt Accession #: P15086; O60834; Q53XJ0; Q96BQ8
• Molecular Weight: Molecular Mass: The recombinant human CPB1 consists of 413 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 45 kDa band
• NCBI Official Full Name: carboxypeptidase B preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B1
• NCBI Official Symbol: CPB1
• NCBI Official Synonym Symbols: CPB; PASP; PCPB
• NCBI Protein Information: carboxypeptidase B
• UniProt Protein Name: Carboxypeptidase B
• UniProt Gene Name: CPB1
• UniProt Synonym Gene Names: CPB; PCPB; PASP
• UniProt Entry Name: CBPB1_HUMAN