CD106 (VCAM1) Active Protein | CD106 active protein
Human CD106 Recombinant (VCAM1)
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
CD106 (VCAM1); N/A; Human CD106 Recombinant (VCAM1); CD106 active protein
Host
HEK293 cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant CD106 is supplied as a 0.2 um filtered PBS solution, pH7.2.
Sequence Length
698
Source Note
Optimized DNA sequence encoding Human extracellular domain of Human CD106 including a C-terminal 6His tag was expressed in HEK293 cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Activity
The activity of recombinant VCAM1 was tested by immobilized recombinant CD106 (10ug/ml) ability to support adhesion of U937 human histiocytic lymphoma cells (5X104 cells/well), 70% will adhere after 60 minutes at 37°C.
Preparation and Storage
Recombinant CD106, as supplied, can be stored in working aliquots at 2°C - 8°C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Product Categories/Family for CD106 active protein
References
• differential engagement of modules 1 and 4 of vascular cell adhesion molecule-1 (CD106) by integrins 41 (cd49d/29) and m2 (cd11b/18) of eosinophils .J. Biol. Chem., Oct 2006; 281: 32175 - 32187.
• novel expression of vascular cell adhesion molecule-1 (CD106) by squamous epithelium in experimental acute graft-versus-host disease .Am. J. Pathol., Sep 2002; 161: 763 - 770.
• vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor .Blood, Sep 2001; 98: 1289 - 1297.
• rheumatoid arthritis synovial stromal cells inhibit apoptosis and up-regulate bcl-xl expression by b cells in a cd49/cd29-CD106-dependent mechanism .J. Immunol., Jan 2000; 164: 1110 - 1116.
• thrombin-activated human endothelial cells support monocyte adhesion in vitro following expression of intercellular adhesion molecule-1 (icam-1; cd54) and vascular cell adhesion molecule-1 (vcam-1; CD106) .Blood, Aug 1998; 92: 1259 - 1267.
• novel expression of vascular cell adhesion molecule-1 (CD106) by squamous epithelium in experimental acute graft-versus-host disease .Am. J. Pathol., Sep 2002; 161: 763 - 770.
• vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor .Blood, Sep 2001; 98: 1289 - 1297.
• rheumatoid arthritis synovial stromal cells inhibit apoptosis and up-regulate bcl-xl expression by b cells in a cd49/cd29-CD106-dependent mechanism .J. Immunol., Jan 2000; 164: 1110 - 1116.
• thrombin-activated human endothelial cells support monocyte adhesion in vitro following expression of intercellular adhesion molecule-1 (icam-1; cd54) and vascular cell adhesion molecule-1 (vcam-1; CD106) .Blood, Aug 1998; 92: 1259 - 1267.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Weight Note: Recombinant CD106 (VCAM1) is a monomer protein consisting of 689 amino acid residue subunits, due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE.
NCBI Official Full Name
vascular cell adhesion protein 1 isoform a
UniProt Protein Name
Vascular cell adhesion protein 1
UniProt Gene Name
VCAM1
UniProt Synonym Gene Names
L1CAM; V-CAM 1; VCAM-1
UniProt Entry Name
VCAM1_HUMAN
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Product Notes
The CD106 vcam1 (Catalog #AAA14416) is an Active Protein produced from HEK293 cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD106 (VCAM1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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