ELISA
(Figure 2. ELISA plate pre-coated by 2ug/ml (100ul/well) Human B7-H3, mFc-His tagged protein (PME100012) can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25ng/ml.)
B7-H3 protein
Human B7-H3 Protein, mFc-His Tag
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
B7-H3; N/A; Human B7-H3 Protein, mFc-His Tag; CD276; B7 homolog 3; B7-H3 protein
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5 %-19 % trehalose is added as protectants before lyophilization.
Sequence Positions
B7-H3 (Leu29-Pro245)+mFc (Pro99-Lys330)
Species
Human
Vector and Tag
C-Mouse Fc and 6xHis tag
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
ELISA
(Figure 2. ELISA plate pre-coated by 2ug/ml (100ul/well) Human B7-H3, mFc-His tagged protein (PME100012) can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25ng/ml.)
ELISA
(Figure 2. ELISA plate pre-coated by 2ug/ml (100ul/well) Human B7-H3, mFc-His tagged protein (PME100012) can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25ng/ml.)
SDS-PAGE
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human B7-H3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.)
Related Product Information for B7-H3 protein
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Similar Products
Product Notes
The B7-H3 (Catalog #AAA268934) is a Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is B7-H3 (Leu29-Pro245)+mFc (Pro99-Lys330). It is sometimes possible for the material contained within the vial of "B7-H3, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.