COVID 19 Spike RBD Coronavirus Active Protein | COVID-19 active protein
SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)
Purity
>95% as determined by SDS-PAGE.Synonyms
COVID 19 Spike RBD Coronavirus; N/A; SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike RBD; Receptor Binding Domain; COVID-19 active protein
Host
HEK293 Cells
Source: 2019nCoV
Source: 2019nCoV
Purity/Purification
>95% as determined by SDS-PAGE.
Form/Format
Liquid or lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Predicted N terminal
Arg 319
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fctag) at 10 u g/mL (100 u L/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, HisTag)(ZD2019-P11), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag)(ZD2019-P11)is 50-100 ng/mL.
Protein Construction
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD)(YP_009724390.1) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus.
Molecular Mass
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag) consists of234 amino acids and predicts a molecular mass of 23.54 kDa
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Samples are stable for 12 months from date of receipt at -20 degree C to -80 degree C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thawcycles
Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thawcycles
Related Product Information for COVID-19 active protein
Description:
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bindto certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-relatedcell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential forboth host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to beessential in the binding of the virus to the host cell at the advent of the infection process.
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bindto certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-relatedcell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential forboth host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to beessential in the binding of the virus to the host cell at the advent of the infection process.
References
References:
Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S)glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein ofSARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X,et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S)glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. Du L, et al. (2009) The spike protein ofSARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X,et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
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Product Notes
The COVID-19 (Catalog #AAA268888) is an Active Protein produced from HEK293 Cells Source: 2019nCoV and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 Spike RBD Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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