IHC (Immunohistochemisry)
(Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (AAA40912, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Rabbit COVID 19 Membrane Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Membrane Antibody
Reactivity
Virus
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography purified via peptide column
Synonyms
COVID 19 Membrane Coronavirus, Antibody; SARS-CoV-2 (COVID-19) Membrane Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Membrane; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography purified via peptide column
Form/Format
Liquid
SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Anti-SARS-CoV-2 (COVID-19) Membrane antibody (MBS154665) was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) Membrane protein.
The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Membrane protein.
The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Membrane protein.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Matrix protein: (93%)
Conjugation
Unconjugated
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
IHC (Immunohistochemisry)
(Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (AAA40912, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
IHC (Immunohistochemisry)
(Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Membrane antibody (AAA40912, 0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Membrane protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
WB (Western Blot)
(Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane Protein Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein. Antibodies: SARS-CoV-2 (COVID-19) membrane, AAA40912, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1:0.25 ug/mL and Lane 2:0.5 ug/mL)
WB (Western Blot)
(Western Blot Validation with SARS-CoV-2 (COVID-19) Membrane Protein Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) membrane protein. Antibodies: SARS-CoV-2 (COVID-19) membrane, AAA40912, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1:0.25 ug/mL and Lane 2:0.5 ug/mL)
ELISA
(Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19 Membrane ProteinAntibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, AAA40912. A direct ELISA was performed using SARS-COV-2 membrane protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 3000 ng/mL.)
ELISA
(Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19 Membrane ProteinAntibodies: SARS-CoV-2 (COVID-19) Membrane Antibody, AAA40912. A direct ELISA was performed using SARS-COV-2 membrane protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Membrane antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 3000 ng/mL.)
Product Categories/Family for anti-COVID-19 antibody
References
Zhu et al. N Engl J Med. 2020 Feb 20;382(8):727-733.
Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575
Neuman et al. J Struct Biol. 2011;174(1):11-22.
Liu et al. J Infect Dis. 2010;202(8):1171-80.
Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575
Neuman et al. J Struct Biol. 2011;174(1):11-22.
Liu et al. J Infect Dis. 2010;202(8):1171-80.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
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Product Notes
The COVID-19 (Catalog #AAA40912) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Membrane Antibody reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Membrane Coronavirus can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Membrane Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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