Application Data
(Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10 ug/mL (100 uL/well) can bind TDGF1-Fc, the EC50 of human TDGF1-Fc is 6.0-14 ng/mL.)
Cripto Active Protein | TDGF1 active protein
Cripto Protein, Human, Recombinant (hFc Tag)
Gene Names
TDGF1; CR; CRGF; CRIPTO
Purity
>95% as determined by SDS-PAGE
Synonyms
Cripto; N/A; Cripto Protein, Human, Recombinant (hFc Tag); Human Cripto Protein (Fc Tag); CR Protein, Human; CRGF Protein, Human; CRIPTO Protein, Human; teratocarcinoma-derived growth factor 1; TDGF1 active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Thr172
Species
Human
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Leu 31
Tag
C-human IgG1-Fc
Protein Construction
A DNA sequence encoding the human TDGF1 (NP_003203.1)(Met1-Thr172) was expressed with the Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10ug/mL (100uL/well) can bind TDGF1-Fc. The EC50 of human TDGF1-Fc is 6.0-14ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10 ug/mL (100 uL/well) can bind TDGF1-Fc, the EC50 of human TDGF1-Fc is 6.0-14 ng/mL.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10 ug/mL (100 uL/well) can bind TDGF1-Fc, the EC50 of human TDGF1-Fc is 6.0-14 ng/mL.)
SDS-PAGE
SDS-PAGE
Related Product Information for TDGF1 active protein
Background: Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal-distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal-distal asymmetry into an orthogonal anterior-posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.
Product Categories/Family for TDGF1 active protein
References
Calvanese L, et al. (2006) Solution structure of mouse Cripto CFC domain and its inactive variant Trp107Ala. J Med Chem. 49 (24): 7054-62.Lonardo E, et al. (2010) A small synthetic cripto blocking Peptide improves neural induction, dopaminergic differentiation, and functional integration of mouse embryonic stem cells in a rat model of Parkinson's disease. Stem Cells. 28 (8): 1326-37.Chambery A, et al. (2009) Qualitative and quantitative proteomic profiling of cripto(-/-) embryonic stem cells by means of accurate mass LC-MS analysis. J Proteome Res. 8 (2): 1047-58.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,169 Da
NCBI Official Full Name
teratocarcinoma-derived growth factor 1 isoform 1
NCBI Official Synonym Full Names
teratocarcinoma-derived growth factor 1
NCBI Official Symbol
TDGF1
NCBI Official Synonym Symbols
CR; CRGF; CRIPTO
NCBI Protein Information
teratocarcinoma-derived growth factor 1
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Product Notes
The TDGF1 (Catalog #AAA257949) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Thr17 2. It is sometimes possible for the material contained within the vial of "Cripto, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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