IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human CRIPTO (TDGF1) Polyclonal Antibody | anti-TDGF1 antibody
CRIPTO (TDGF1) Antibody (N-term)
Gene Names
TDGF1; CR; CRGF; CRIPTO
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
This anitbody was purified through a Protein A column, followed by peptide affinity purification.
Synonyms
CRIPTO (TDGF1), Antibody; CRIPTO (TDGF1) Antibody (N-term); Teratocarcinoma-derived growth factor 1; Cripto-1 growth factor; CRGF; Epidermal growth factor-like cripto protein CR1; TDGF1; CRIPTO; anti-TDGF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CRIPTO (TDGF1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-48 amino acids from the N-terminal region of human CRIPTO (TDGF1).
Purity/Purification
This anitbody was purified through a Protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
18-48
Sequence Length
172
Applicable Applications for anti-TDGF1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
GPI-anchored cell membrane protein involvedin Nodal signaling. Cell-associated TDGF1acts as a Nodal coreceptor in cis. Shedding ofTDGF1 by TMEM8A modulates Nodal signalingby allowing soluble TDGF1 to act as a Nodalcoreceptor on other cells (PubMed: ). Could playa role in the determination of the epiblasticcells that subsequently give rise to themesoderm (PubMed: )
Cellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Released from the cell membrane by GPI cleavage.
Tissue Location
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of CRIPTO (TDGF1) Antibody (N-term) in Jurkat cell line lysates (35ug/lane). TDGF1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CRIPTO (TDGF1) Antibody (N-term) in Jurkat cell line lysates (35ug/lane). TDGF1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-TDGF1 antibody
TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers. This protein could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. It is preferentially expressed in gastric and colorectal carcinomas compared to their normal counterparts.
Product Categories/Family for anti-TDGF1 antibody
References
Parisi, S., et al., J. Cell Biol. 163(2):303-314 (2003).
Gray, P.C., et al., Proc. Natl. Acad. Sci. U.S.A. 100(9):5193-5198 (2003).
Yan, Y.T., et al., Mol. Cell. Biol. 22(13):4439-4449 (2002).
de la Cruz, J.M., et al., Hum. Genet. 110(5):422-428 (2002).
Dono, R., et al., Am. J. Hum. Genet. 49(3):555-565 (1991).
Gray, P.C., et al., Proc. Natl. Acad. Sci. U.S.A. 100(9):5193-5198 (2003).
Yan, Y.T., et al., Mol. Cell. Biol. 22(13):4439-4449 (2002).
de la Cruz, J.M., et al., Hum. Genet. 110(5):422-428 (2002).
Dono, R., et al., Am. J. Hum. Genet. 49(3):555-565 (1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21169
NCBI Official Full Name
teratocarcinoma-derived growth factor 1 isoform 2
NCBI Official Synonym Full Names
teratocarcinoma-derived growth factor 1
NCBI Official Symbol
TDGF1
NCBI Official Synonym Symbols
CR; CRGF; CRIPTO
NCBI Protein Information
teratocarcinoma-derived growth factor 1
UniProt Protein Name
Teratocarcinoma-derived growth factor 1
UniProt Gene Name
TDGF1
UniProt Synonym Gene Names
CRIPTO; CRGF
UniProt Entry Name
TDGF1_HUMAN
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Product Notes
The TDGF1 tdgf1 (Catalog #AAA289269) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 18-48. The CRIPTO (TDGF1) Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CRIPTO (TDGF1) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TDGF1 tdgf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRIPTO (TDGF1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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