TIE2 Active Protein | TEK active protein

TIE2 Protein, Human, Recombinant (ECD, His & hFc Tag)

Cat. Num. AAA257917

• Gene Names: TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TIE2; N/A; TIE2 Protein, Human, Recombinant (ECD, His & hFc Tag); Human Tie2/CD202b Protein (ECD, His & Fc Tag); CD202B Protein, Human; TIE-2 Protein, Human; TIE2 Protein, Human; VMCM Protein, Human; VMCM1 Protein, Human; TEK tyrosine kinase, endothelial; TEK active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys745

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10ug/ml (100uL/well) can bind Human Tie2/Fc chimera with a range of 0.2-20ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 23
• Tag: C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met1-Lys745) of human Tie2 (AAA61139.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 ug/ml (100 ul/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 ug/ml.)

SDS-PAGE

Related Product Information for TEK active protein

Background: TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Product Categories/Family for TEK active protein

Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets Hemangioblast Markers

References

Jones N, et al. (1998) The Tek/Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108.Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27.Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK/TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.

NCBI and Uniprot Product Information

• NCBI GI #: 88758596
• NCBI GeneID: 7010
• NCBI Accession #: NP_000450.2
• NCBI GenBank Nucleotide #: NM_000459.3
• UniProt Accession #: Q02763; Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2
• Molecular Weight: 125,830 Da
• NCBI Official Full Name: angiopoietin-1 receptor
• NCBI Official Synonym Full Names: TEK tyrosine kinase, endothelial
• NCBI Official Symbol: TEK
• NCBI Official Synonym Symbols: TIE2; VMCM; TIE-2; VMCM1; CD202B
• NCBI Protein Information: angiopoietin-1 receptor; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase wi
• UniProt Protein Name: Angiopoietin-1 receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN