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product-image-AAA129482_SDS_PAGE13.jpg SDS-PAGE (Figure 2. Human MBP-AGTR1-Nanodisc with N-MBP Tag, C-DYKDDDDK Tag on SDS-PAGE)

MBP-AGTR1 Protein | AGTR1 protein

Human MBP-AGTR1 full length protein-synthetic nanodisc

Gene Names
AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1A; AGTR1B; AT2R1A; AT2R1B
Synonyms
MBP-AGTR1; N/A; Human MBP-AGTR1 full length protein-synthetic nanodisc; AG2S; AGTR1B; AT1; AT1AR; AT1B; AT1BR; AT1R; AT2R1; HAT1R; AGTR1 protein
Ordering
Host
HEK293
Form/Format
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5%-8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Lyophilized from PBS. Normally 5%-
Tag
N-MBP, C-DYKDDDDK
Preparation and Storage
Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

SDS-PAGE

(Figure 2. Human MBP-AGTR1-Nanodisc with N-MBP Tag, C-DYKDDDDK Tag on SDS-PAGE)

product-image-AAA129482_SDS_PAGE13.jpg SDS-PAGE (Figure 2. Human MBP-AGTR1-Nanodisc with N-MBP Tag, C-DYKDDDDK Tag on SDS-PAGE)

ELISA

(Figure 1. Elisa plates were pre-coated with N-MBP Tag, C-DYKDDDDK Tag MBP-AGTR1-Nanodisc (0.2ug/per well). Serial diluted anti-DYKDDDDK monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-DYKDDDDK monoclonal antibody binding with MBP-AGTR1-Nanodisc is 4.309ng/ml.)

product-image-AAA129482_ELISA15.jpg ELISA (Figure 1. Elisa plates were pre-coated with N-MBP Tag, C-DYKDDDDK Tag MBP-AGTR1-Nanodisc (0.2ug/per well). Serial diluted anti-DYKDDDDK monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-DYKDDDDK monoclonal antibody binding with MBP-AGTR1-Nanodisc is 4.309ng/ml.)
Related Product Information for AGTR1 protein
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
185
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,061 Da
NCBI Official Full Name
type-1 angiotensin II receptor isoform 1
NCBI Official Synonym Full Names
angiotensin II receptor, type 1
NCBI Official Symbol
AGTR1
NCBI Official Synonym Symbols
AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1A; AGTR1B; AT2R1A; AT2R1B
NCBI Protein Information
type-1 angiotensin II receptor; type-1B angiotensin II receptor
UniProt Protein Name
Type-1 angiotensin II receptor
UniProt Gene Name
AGTR1
UniProt Synonym Gene Names
AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1
UniProt Entry Name
AGTR1_HUMAN

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Product Notes

The AGTR1 agtr1 (Catalog #AAA129482) is a Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MBP-AGTR1, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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