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product-image-AAA99290_SC13.jpg Standard Curve (Sample)

Human Hemojuvelin ELISA Kit | HFE2 elisa kit

Human Hemojuvelin ELISA Kit

Gene Names
Hfe2; HJV; DL-M; Rgmc; AI414844; AI789733; hemojuvelin; 2310035L15Rik; 5230400G09Rik
Reactivity
Human
Synonyms
Hemojuvelin; N/A; Human Hemojuvelin ELISA Kit; HJV/HFE2/Hemojuvelin/Hemochromatosis Type 2)/RGM-C/DL-M/JH/haemojuvelin/HJVHemochromatosis type 2 protein/repulsive guidance molecule c/RGMCRGM domain family member C; HFE2 elisa kit
Ordering
Reactivity
Human
Sequence Length
420
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

product-image-AAA99290_SC13.jpg Standard Curve (Sample)

Standard Curve (Sample)

product-image-AAA99290_SC15.jpg Standard Curve (Sample)
Related Product Information for HFE2 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HJVantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HJV antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HJV amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman HJVcan be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,648 Da
NCBI Official Full Name
hemojuvelin
NCBI Official Synonym Full Names
hemochromatosis type 2 (juvenile)
NCBI Official Symbol
Hfe2
NCBI Official Synonym Symbols
HJV; DL-M; Rgmc; AI414844; AI789733; hemojuvelin; 2310035L15Rik; 5230400G09Rik
NCBI Protein Information
hemojuvelin
UniProt Protein Name
Hemojuvelin
UniProt Gene Name
Hfe2
UniProt Synonym Gene Names
Rgmc
UniProt Entry Name
RGMC_MOUSE

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Product Notes

The Human HFE2 hfe2 (Catalog #AAA99290) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA99290 ELISA Kit recognizes Human HFE2. It is sometimes possible for the material contained within the vial of "Hemojuvelin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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