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IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cells using HIF-1? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Mouse HIF-1alpha Monoclonal Antibody | anti-HIF-1alpha antibody
HIF-1alpha Monoclonal Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Monkey
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
HIF-1alpha, Antibody; HIF-1alpha Monoclonal Antibody; Hypoxia-inducible factor 1-alpha; HIF1A; BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma; anti-HIF-1alpha antibody
Host
Mouse
Reactivity
Human, Mouse, Monkey
Clonality
Monoclonal
Specificity
HIF-1alpha Monoclonal Antibody detects endogenous levels of HIF-1alpha protein.
Purity/Purification
Affinity purification
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-HIF-1alpha antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human HIF-1alpha expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cells using HIF-1? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cells using HIF-1? Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) with DAB staining using HIF-1? Monoclonal Antibody.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) with DAB staining using HIF-1? Monoclonal Antibody.)
Application Data
(Sun. Lei. et al. "Beclin-1-independent autophagy mediates programmed cancer cell death through interplays with endoplasmic reticulum and/or mitochondria in colbat chloride-induced hypoxia." American journal of cancer research 5.9 (2015): 2626.)
Application Data
(Sun. Lei. et al. "Beclin-1-independent autophagy mediates programmed cancer cell death through interplays with endoplasmic reticulum and/or mitochondria in colbat chloride-induced hypoxia." American journal of cancer research 5.9 (2015): 2626.)
WB (Western Blot)
(Western Blot analysis using HIF-1? Monoclonal Antibody against Cos7 (1). HeLa (2). Jurkat (3). RAJI (4) and NIH/3T3 (5) cell lysate.)
WB (Western Blot)
(Western Blot analysis using HIF-1? Monoclonal Antibody against Cos7 (1). HeLa (2). Jurkat (3). RAJI (4) and NIH/3T3 (5) cell lysate.)
Related Product Information for anti-HIF-1alpha antibody
hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Product Categories/Family for anti-HIF-1alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,670 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
NCBI Protein Information
hypoxia-inducible factor 1-alpha; HIF-1-alpha; member of PAS protein 1; ARNT interacting protein; ARNT-interacting protein; member of PAS superfamily 1; hypoxia-inducible factor1alpha; PAS domain-containing protein 8; basic-helix-loop-helix-PAS protein MO
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The HIF-1alpha hif1a (Catalog #AAA320102) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIF-1alpha Monoclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's HIF-1alpha can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HIF-1alpha hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF-1alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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