Standard Curve (Sample)
Human HLA class II histocompatibility antigen, DR beta 5 chain ELISA Kit | HLA-DRB5 elisa kit
Human HLA class II histocompatibility antigen, DR beta 5 chain ELISA Kit
Gene Names
HLA-DRB5; HLA-DRB
Reactivity
Human
Synonyms
HLA class II histocompatibility antigen, DR beta 5 chain; N/A; Human HLA class II histocompatibility antigen, DR beta 5 chain ELISA Kit; DR beta-5; DR2-beta-2; Dw2; MHC class II antigen DRB5; HLA-DRB5; HLA-DRB5 elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural Human DRB5. No significant cross-reactivity or interference was observed.
Sequence Length
266
Assay Type
Competitive
Detection Range
1.56-100 ng/mL
Sensitivity
0.16ng/ml
Intra-Assay CV
<=6.3%
Inter-Assay CV
<=9.4%
Recovery
101%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HLA-DRB5 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human hla class ii histocompatibility antigen, dr beta 5 chain,DRB5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DRB5, During the reaction, DRB5 in the sample or standard competes with a fixed amount of biotin-labeled DRB5 for sites on a pre-coated Monoclonal antibody specific to DRB5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DRB5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DRB5, During the reaction, DRB5 in the sample or standard competes with a fixed amount of biotin-labeled DRB5 for sites on a pre-coated Monoclonal antibody specific to DRB5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DRB5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,056 Da
NCBI Official Full Name
major histocompatibility complex, class II, DR beta 5
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DR beta 5
NCBI Official Symbol
HLA-DRB5
NCBI Official Synonym Symbols
HLA-DRB
NCBI Protein Information
major histocompatibility complex, class II, DR beta 5
UniProt Protein Name
HLA class II histocompatibility antigen, DR beta 5 chain
UniProt Gene Name
HLA-DRB5
UniProt Entry Name
DRB5_HUMAN
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Product Notes
The Human HLA-DRB5 hla-drb5 (Catalog #AAA190698) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA190698 ELISA Kit recognizes Human HLA-DRB5. It is sometimes possible for the material contained within the vial of "HLA class II histocompatibility antigen, DR beta 5 chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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