IHC (Immunohistochemisry)
(DAB staining on IHCP;Sample: Human Spinal cord Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Mouse anti-Human, Pig High Mobility Group Protein 1 (HMGB1) Monoclonal Antibody | anti-HMGB1 antibody
Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
High Mobility Group Protein 1 (HMGB1), Antibody; Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1); HMGB1/High Mobility Group Protein 1; HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; anti-HMGB1 antibody
Host
Mouse
Reactivity
Human, Pig
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
D6
Specificity
The antibody is a mouse monoclonal antibody raised against HMGB1. It has been selected for its ability to recognize HMGB1 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-HMGB1 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Prokaryotic Recombinant proteins/RP
Sequence of Immunogen
Met1~Glu215
Conjugation
Unconjugated
Preparation and Storage
Store at 2 degree C to 8 degree C for frequent use, 20 degree C for 24 months
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemisry)
(DAB staining on IHCP;Sample: Human Spinal cord Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
IHC (Immunohistochemisry)
(DAB staining on IHCP;Sample: Human Spinal cord Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
IHC (Immunohistochemistry)
(DAB staining on IHCP; Sample: Human Cerebellum Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
IHC (Immunohistochemistry)
(DAB staining on IHCP; Sample: Human Cerebellum Tissue; Primary Ab: 10ug/ml Mouse AntiHuman HMG1 Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Product Categories/Family for anti-HMGB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
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Product Notes
The HMGB1 hmgb1 (Catalog #AAA151530) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's High Mobility Group Protein 1 (HMGB1) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HMGB1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "High Mobility Group Protein 1 (HMGB1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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