SDS-PAGE
(SDS-PAGE of 65kDa M. Bovis Hsp65 protein (AAA17808).)
Hsp65 Recombinant Protein | groEL recombinant protein
Recombinant M. bovis HSP65, native sequence
Reactivity
Mycobacterium bovis BCG
Applications
Western Blot
Purity
Multi-Step Chromatography
Synonyms
Hsp65; N/A; Recombinant M. bovis HSP65, native sequence; 60kD chaperonin 2; Antigen A; Cell wall protein A; groEL; GroEL2; GroL2; M. Tuberculosis cell wall protein A; M. Tuberculosis Hsp65; Protein Cpm60 2; groEL recombinant protein
Host
Mycobacterium bovis BCG
Reactivity
Mycobacterium bovis BCG
Purity/Purification
Multi-Step Chromatography
Form/Format
20mM Tris, 150mM NaCl, 10% glycerol.
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for groEL recombinant protein
Western Blot (WB) Control in vivo cytotoxicity assay
Certificate of Analysis
This product has been certified >90% pure using SDS-PAGE analysis.
Expression System
E Coli
Cellular Localization
Cytoplasm
Research Area
Heat Shock
Function
Hsp65 isolated from Mycobacterium bovis BCG, is a member of the hsp60 family of heat shock proteins (2, 3). Hsp60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, Hsp60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. Hsp60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically, sequence comparison of Hsp65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG Hsp65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6).
Preparation and Storage
-20°C ; 1 year. Avoid freeze / thaw cycle.
SDS-PAGE
(SDS-PAGE of 65kDa M. Bovis Hsp65 protein (AAA17808).)
SDS-PAGE
(SDS-PAGE of 65kDa M. Bovis Hsp65 protein (AAA17808).)
Product Categories/Family for groEL recombinant protein
References
1. Koll H., et al. (1992) Cell 68: 1163-1175. 2. Thole J.E.R., et al. (1985) Infect. Immuno. 50: 800- 806. 3. Thole J.E.R., et al., (1987) Infect. Immuno. 55: 1466- 1475. 4. Shinnick T.M. Sweetser D., Thole J., van Embden J. and Young R.A. (1987) Infect. Immuno. 55: 1932-1935. 5. Van Eden W., et al. (1988) Nature 331: 171-178. 6. Cobelens P.M., et al. (2002) Rheumatology 41: 775- 779.
1. Adamus, G. et. al. (2013). Detection of autoantibodies against heat shock proteins and collapsin response
mediator proteins in autoimmune retinopathy. BMC Ophthalmology 2013, 13:48 doi:10.1186/1471-2415-13-48
1. Adamus, G. et. al. (2013). Detection of autoantibodies against heat shock proteins and collapsin response
mediator proteins in autoimmune retinopathy. BMC Ophthalmology 2013, 13:48 doi:10.1186/1471-2415-13-48
NCBI and Uniprot Product Information
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Product Notes
The groEL (Catalog #AAA17808) is a Recombinant Protein produced from Mycobacterium bovis BCG and is intended for research purposes only. The product is available for immediate purchase. The Recombinant M. bovis HSP65, native sequence reacts with Mycobacterium bovis BCG and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) Control in vivo cytotoxicity assay. Researchers should empirically determine the suitability of the groEL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp65, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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