SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW 96kDa)
HSP70 recombinant protein
HSP70 recombinant protein
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A
Applications
Western Blot
Purity
>90%
Synonyms
HSP70; N/A; HSP70 recombinant protein; HSPA1A; HSP70-1; HSP72; HSPA1; HSPA1B. DAQB-147D11.1
Host
E Coli
Purity/Purification
>90%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.2 ug/ul (varies by lot)
Sequence Length
2445
Applicable Applications for HSP70 recombinant protein
WB (Western Blot), ATPase Assay
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant full-length human HSP70 was expressed in E. coli cells
Accession Number
NM 005345
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Stability: 1 year at -70 degree C from date of shipment.
SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW 96kDa)
SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW 96kDa)
Related Product Information for HSP70 recombinant protein
Recombinant full-length human HSP70 was expressed in E. coli cells using an N-terminal GST tag.
Scientific Background: HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli.
Scientific Background: HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli.
Product Categories/Family for HSP70 recombinant protein
References
1. Moran, L A. et al: The major heat-shock protein (hsp70) gene family: related sequences in mouse, Drosophila, and yeast. Can J Biochem Cell Biol. 1983 Jun;61(6):488-99.
2. Pelham, H R: Hsp70 accelerates the recovery of nucleolar morphology after heat shock. EMBO J. 1984 Dec 20;3(13):3095-100.
2. Pelham, H R: Hsp70 accelerates the recovery of nucleolar morphology after heat shock. EMBO J. 1984 Dec 20;3(13):3095-100.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~96 kDa
NCBI Official Full Name
Homo sapiens heat shock 70kDa protein 1A (HSPA1A), mRNA
NCBI Official Synonym Full Names
heat shock 70kDa protein 1A
NCBI Official Symbol
HSPA1A
NCBI Official Synonym Symbols
HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A
NCBI Protein Information
heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; dnaK-type molecular chaperone HSP70-1
UniProt Protein Name
Heat shock 70 kDa protein 1A/1B
UniProt Gene Name
HSPA1A
UniProt Synonym Gene Names
HSPA1; HSX70; HSP70-1/HSP70-2
UniProt Entry Name
HSP71_HUMAN
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Product Notes
The HSP70 hspa1a (Catalog #AAA72885) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||E.coli!!Source Note||Recombinant full-length human HSP70 was expressed in E. coli cells!!Accession Number||NM 005345. AAA Biotech's HSP70 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ATPase Assay. Researchers should empirically determine the suitability of the HSP70 hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP70, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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