SDS-PAGE
HtrA2/Omi recombinant protein
HtrA2/Omi, 134-458aa, Human, His tag, E Coli
Gene Names
HTRA2; OMI; MGCA8; PARK13; PRSS25
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
HtrA2/Omi; N/A; HtrA2/Omi, 134-458aa, Human, His tag, E Coli; High temperature requirement protein A2; HTRA2; OMI; PRSS25; EC 3.4.21.108; HtrA2; Omi stress-regulated endoprotease; Serine proteinase OMI; Serine protease 25; Serine protease HTRA2; mitochondrial; HtrA2/Omi recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0), 50 mM NaCl, 1 mM DTT, 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGFVVAADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT LPLGRSADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EYIQTDAAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRGEKKNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHKVILGSPA HRAGLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGRETLTLYV TPEVTEGSHH HHHH
Sequence Length
458
Applicable Applications for HtrA2/Omi recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for HtrA2/Omi recombinant protein
HtrA2/Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death. Mature form of HtrA2/Omi (residues 134-458) was overexpressed in E Coli, and purified by conventional column chromatography techniques.
Product Categories/Family for HtrA2/Omi recombinant protein
References
Savopoulos JW., et al., (2000) Protein Expr. Purif. 19, 227-34; Gray CW, et al., (2000) Eur. J. Biochem. 267, 5699-710 ; Martins LM, et al., (2002) J. Biol. Chem. 277, 439-44; Van Loo G, et al., (2002) Cell Death Differ. 9, 20-60;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36kDa (334aa), confirmed by MALDI-TOF.
NCBI Official Full Name
serine protease HTRA2, mitochondrial isoform 1 preproprotein
NCBI Official Synonym Full Names
HtrA serine peptidase 2
NCBI Official Symbol
HTRA2
NCBI Official Synonym Symbols
OMI; MGCA8; PARK13; PRSS25
NCBI Protein Information
serine protease HTRA2, mitochondrial
UniProt Protein Name
Serine protease HTRA2, mitochondrial
UniProt Gene Name
HTRA2
UniProt Synonym Gene Names
OMI; PRSS25; HtrA2
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Product Notes
The HtrA2/Omi htra2 (Catalog #AAA48468) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's HtrA2/Omi can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HtrA2/Omi htra2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGFVVAADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT LPLGRSADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EYIQTDAAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRGEKKNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHKVILGSPA HRAGLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGRETLTLYV TPEVTEGSHH HHHH. It is sometimes possible for the material contained within the vial of "HtrA2/Omi, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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