IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Alpha actin 1 antibody 3G8C7A6 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3G8C7A6 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human Alpha actin 1 Antibody | anti-ACTA1 antibody
Anti-Alpha actin 1 [3G8C7A6]
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Purified. Protein A affinity purified
Synonyms
Alpha actin 1; N/A; Anti-Alpha actin 1 [3G8C7A6]; Actin; ACTA1; ACTA; Actin alpha skeletal muscle; Alpha-actin-1; Muscle specific actin; MSA; HHF35; CGMCNO.22312; anti-ACTA1 antibody
Reactivity
Human
Isotype
Mouse IgG2b kappa
Clone Number
3G8C7A6
Specificity
This antibody binds actin. Actin is a type of spherical multi-functional protein that forms a microfilament. Actin is widely existent in eukaryotic organisms, and there is a major component constituting the muscle segment and the cell skeleton in the cytoplasm and cellular nucleus of the intramuscular cell. Muscle specific actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. In addition to participating in the normal morphology of maintaining cells, actin also participates in the formation, adhesion, migration, apoptosis, material transport, transmembrane information transfer, and receptor aggregation.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ACTA1 antibody
IHC (Immunohistochemistry), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Alpha actin 1 antibody 3G8C7A6 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3G8C7A6 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Alpha actin 1 antibody 3G8C7A6 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3G8C7A6 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western blot using anti-Alpha actin 1 antibody 3G8C7A6 . HEK293 (A) (1ug/ml), HeLa (B) (0.1ug/ml) cells lysate, and human skeletal muscle (C) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 3G8C7A6 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-Alpha actin 1 antibody 3G8C7A6 . HEK293 (A) (1ug/ml), HeLa (B) (0.1ug/ml) cells lysate, and human skeletal muscle (C) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 3G8C7A6 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Alpha actin 1 antibody 3G8C7A6 . Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 3G8C7A6 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Alpha actin 1 antibody 3G8C7A6 . Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 3G8C7A6 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-ACTA1 antibody
The original antibody was generated by immunizing BALB/c mice with a recombinant human actin protein.
Product Categories/Family for anti-ACTA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,051 Da
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle; nemaline myopathy type 3
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
Similar Products
Product Notes
The ACTA1 acta1 (Catalog #AAA72365) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha actin 1 [3G8C7A6] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha actin 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha actin 1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.