anti-Human CD142 Antibody | anti-CD142 antibody

Anti-Hu CD142 APC

Cat. Num. AAA129303

• Gene Names: F3; TF; TFA; CD142
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD142; N/A; Anti-Hu CD142 APC; coagulation factor III, tissue factor; F3; tissue factor; tissue thromboplastin; coagulation factor III; TF; TFA; anti-CD142 antibody

• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: HTF-1
• Specificity: The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD142 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Human brain tissue factor (CD142)
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of A431 cells (red-filled) from human lymfocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood spiked with A431 cells stained using anti-human CD142 (HTF-1) APC antibody (10 ?l reagent / 100 ?l of sample - peripheral whole blood spiked with A431 cells).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood spiked with A431 cells stained using anti-human CD142 (HTF-1) APC antibody (10 ?l reagent / 100 ?l of sample - peripheral whole blood spiked with A431 cells).)

Related Product Information for anti-CD142 antibody

CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.

References

*Bucciarelli P, Martinelli I, Artoni A, Passamonti SM, Previtali E, Merati G, Tripodi A, Mannucci PM: Circulating microparticles and risk of venous thromboembolism. Thromb Res. 2012 May;129(5):591-7, URL: https://www.ncbi.nlm.nih.gov/pubmed/21908018,*Patil R, Ghosh K, Satoskar P, Shetty S: Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PLoS One. 2013 Nov 20;8(11):e81407., URL: https://pubmed.ncbi.nlm.nih.gov/24278436/,*Aass HC, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE: Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79(12):990-9. doi: 10.1002/cyto.a.21147., URL: https://www.ncbi.nlm.nih.gov/pubmed/21990118,*Carson SD, Ross SE, Bach R, Guha A: An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Aug;70(2):490-3., URL: https://www.ncbi.nlm.nih.gov/pubmed/3607285,*Faulk WP, Labarrere CA, Carson SD: Tissue factor: Identification and characterization of cell types in human placentae. Volume 76, Issue 1, 1 July 1990, Pages 86-96., URL: https://www.sciencedirect.com/science/article/pii/S0006497120842633,*Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66., URL: https://pubmed.ncbi.nlm.nih.gov/34120626/,

NCBI and Uniprot Product Information

• NCBI GI #: 296010912
• NCBI GeneID: 2152
• NCBI Accession #: NP_001171567.1
• NCBI GenBank Nucleotide #: NM_001178096.1
• UniProt Accession #: P13726; Q6FHG2; Q86WH4; D3DT47
• Molecular Weight: 33,068 Da
• NCBI Official Full Name: tissue factor isoform 2
• NCBI Official Synonym Full Names: coagulation factor III (thromboplastin, tissue factor)
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; TFA; CD142
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: TF
• UniProt Entry Name: TF_HUMAN