IHC (Immunohiostchemistry)
(Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 ug/ml. Commercially tested by LifeSpan BioSciences.)
Mouse anti-Human CD142 Monoclonal Antibody | anti-CD142 antibody
Anti-Hu CD142 Purified
Gene Names
F3; TF; TFA; CD142
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by protein-A affinity chromatography
Synonyms
CD142, Antibody; Anti-Hu CD142 Purified; F3; tissue factor; tissue thromboplastin; coagulation factor III; TF; TFA; coagulation factor III, tissue factor; anti-CD142 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
HTF-1
Specificity
The mouse monoclonal antibody HTF-1, also known as HTF1-7B8, recognizes an extracellular epitope of CD142 (tissue factor, coagulation factor III), a type I glycoprotein expressed on endothelial cells, monocytes, macrophages, and platelets upon induction by inflammatory mediators, and expressed constitutively by some tumors, the vasculature, placenta, kidney, and central nervous system.
Purity/Purification
Purified by protein-A affinity chromatography
Form/Format
Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD142 antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry)
Immunogen
Human brain tissue factor (CD142)
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.
IHC (Immunohiostchemistry)
(Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 ug/ml. Commercially tested by LifeSpan BioSciences.)
IHC (Immunohiostchemistry)
(Immunohistochemistry staining of human cortex (paraffin-embedded sections) with anti-CD142 (HTF-1), 10 ug/ml. Commercially tested by LifeSpan BioSciences.)
Related Product Information for anti-CD142 antibody
CD142, also known as coagulation factor III, tissue thromboplastin, and tissue factor. It is a transmembrane glycoprotein, which enables cells to initiate the blood coagulation cascades, and Functional Application (FUNC)tions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonFunctional Application (FUNC)tional precursors, this factor is a potent initiator that is fully Functional Application (FUNC)tional when expressed on cell surfaces. It is the only one factor in the coagulation pathway for which a congenital deficiency has not been described.
References
*Bucciarelli P, Martinelli I, Artoni A, Passamonti SM, Previtali E, Merati G, Tripodi A, Mannucci PM: Circulating microparticles and risk of venous thromboembolism. Thromb Res. 2012 May;129(5):591-7, URL: https://www.ncbi.nlm.nih.gov/pubmed/21908018,*Patil R, Ghosh K, Satoskar P, Shetty S: Elevated procoagulant endothelial and tissue factor expressing microparticles in women with recurrent pregnancy loss. PLoS One. 2013 Nov 20;8(11):e81407., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=patil+r+,*Aass HC, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE: Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79(12):990-9. doi: 10.1002/cyto.a.21147., URL: https://www.ncbi.nlm.nih.gov/pubmed/21990118,*Carson SD, Ross SE, Bach R, Guha A: An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Aug;70(2):490-3., URL: https://www.ncbi.nlm.nih.gov/pubmed/3607285
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,068 Da
NCBI Official Full Name
tissue factor isoform 2
NCBI Official Synonym Full Names
coagulation factor III (thromboplastin, tissue factor)
NCBI Official Symbol
F3
NCBI Official Synonym Symbols
TF; TFA; CD142
NCBI Protein Information
tissue factor
UniProt Protein Name
Tissue factor
UniProt Gene Name
F3
UniProt Synonym Gene Names
TF
UniProt Entry Name
TF_HUMAN
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Product Notes
The CD142 f3 (Catalog #AAA129091) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD142 Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD142 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD142 f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD142, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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