FCM/FACS (Flow Cytometry)
(Separation of human CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307b (B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD307b Antibody | anti-CD307b antibody
Anti-Hu CD307b PE
Gene Names
FCRL2; FCRH2; IFGP4; IRTA4; SPAP1; CD307b; SPAP1A; SPAP1B; SPAP1C
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD307b; N/A; Anti-Hu CD307b PE; Fc receptor like 2; FcRL2; FcRH2; IFGP4; IRTA4; SPAP1; anti-CD307b antibody
Reactivity
Human
Isotype
Mouse IgG2a kappa
Clone Number
B24
Specificity
The mouse monoclonal antibody B24 recognizes an extracellular epitope of CD307b, a transmembrane glycoprotein expressed mainly in mature and memory B cells.
Form/Format
PE
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Applicable Applications for anti-CD307b antibody
FCM/FACS (Flow Cytometry)
Immunogen
DNA-immunization followed by a boost with CD307b transfected cells
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307b (B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD19 positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD307b (B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307b(B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD307b(B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307b (B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD307b (B24) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD307b antibody
CD307b is a type I transmembrane glycoprotein of the Fc receptor family. It contains one ITAM motif and two ITIM motifs in its cytoplasmic domain. It is expressed in spleen and lymph nodes in mature B cells and memory B cells. CD307b may be a prognostic marker for chronic lymphocytic leukemia.
References
*Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26, URL: https://www.ncbi.nlm.nih.gov/pubmed/19494275,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,533 Da
NCBI Official Full Name
Fc receptor-like protein 2 isoform 2
NCBI Official Synonym Full Names
Fc receptor-like 2
NCBI Official Symbol
FCRL2
NCBI Official Synonym Symbols
FCRH2; IFGP4; IRTA4; SPAP1; CD307b; SPAP1A; SPAP1B; SPAP1C
NCBI Protein Information
Fc receptor-like protein 2
UniProt Protein Name
Fc receptor-like protein 2
UniProt Gene Name
FCRL2
UniProt Synonym Gene Names
FCRH2; IFGP4; IRTA4; SPAP1; FcR-like protein 2; FcRL2; FcRH2
UniProt Entry Name
FCRL2_HUMAN
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Product Notes
The CD307b fcrl2 (Catalog #AAA129266) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD307b PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD307b can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD307b fcrl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD307b, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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