anti-Human CD32 Antibody | anti-CD32 antibody

Anti-Hu CD32 FITC

Cat. Num. AAA129262

• Gene Names: FCGR2A; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD32; N/A; Anti-Hu CD32 FITC; Fc fragment of IgG receptor IIa; FCG2; FCGR2A; IGFR2; anti-CD32 antibody

• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: 3D3
• Specificity: The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
• Form/Format: FITC; Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 1

• Applicable Applications for anti-CD32 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: purified glycosylated recombinant human FcgammaRIIa2
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD32 (3D3) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD32 (3D3) FITC antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD32 antibody

CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).

References

*Dutertre CA, Bonnin-Gélizé E, Pulford K, Bourel D, Fridman WH, Teillaud JL: A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function. J Leukoc Biol. 2008 Dec;84(6):1511-20, URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pulford+k+bourel+d,*Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 15;4(3):566-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/23933259,*Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA: Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood. 2009 Jul 9;114(2):318-27., URL: https://pubmed.ncbi.nlm.nih.gov/19420354/,*Vely F, Gruel N, Moncuit J, Cochet O, Rouard H, Dare S, Galon J, Sautes C, Fridman WH, Teillaud JL: A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays. Hybridoma. 1997 Dec;16(6):519-28., URL: https://pubmed.ncbi.nlm.nih.gov/9455704/,*Boruchov AM, Heller G, Veri MC, Bonvini E, Ravetch JV, Young JW: Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions. J Clin Invest. 2005 Oct;115(10):2914-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=boruchov+am+heller+g,

NCBI and Uniprot Product Information

• NCBI GI #: 210031822
• NCBI GeneID: 2212
• NCBI Accession #: NP_001129691.1
• NCBI GenBank Nucleotide #: NM_001136219.1
• UniProt Accession #: P12318; Q8WUN1; Q8WW64
• Molecular Weight: 35,001 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-a isoform 1
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIa, receptor (CD32)
• NCBI Official Symbol: FCGR2A
• NCBI Official Synonym Symbols: CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-a; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; Immunoglobulin G Fc receptor II
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-a
• UniProt Gene Name: FCGR2A
• UniProt Synonym Gene Names: CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
• UniProt Entry Name: FCG2A_HUMAN