FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-CD32antibodyAT130-5(AAA72431). RAW264.7cellswerefixedusing2%PFAandstainedwithanti-unknownspecificityantibodyortherabbitIgG1versionofAT130-5(AAA72431,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
CD32 recombinant antibody
Anti-CD32 [AT130-5], Rat IgG1, kappa
Gene Names
Fcgr2b; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
Reactivity
Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD32, Recombinant Antibody; Anti-CD32 [AT130-5], Rat IgG1, kappa; CD32; Anti-CD32 [AT130-5]; FcgammaRIIB; FcgRIIb; CD32B; CDw32; FCG2; FCGR2; FCGR2B; IGFR2; Immunoglobulin G Fc receptor II; Low affinity immunoglobulin gamma Fc region receptor II-b; MGC23887; MGC30032; RP11-5K23.6; CD32 recombinant antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
AT130-5
Specificity
This antibody is specific for murine CD32, an Fc gamma surface receptor protein with a low affinity for IgG antibodies that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD32 recombinant antibody
Blocking, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot), FCM/FACS (Flow Cytometry)
Chimeric Use Statement
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-CD32antibodyAT130-5(AAA72431). RAW264.7cellswerefixedusing2%PFAandstainedwithanti-unknownspecificityantibodyortherabbitIgG1versionofAT130-5(AAA72431,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusingtheanti-CD32antibodyAT130-5(AAA72431). RAW264.7cellswerefixedusing2%PFAandstainedwithanti-unknownspecificityantibodyortherabbitIgG1versionofAT130-5(AAA72431,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000andcellsanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedRAW264.7cellswithanti-CD32antibodyAT130-5(AAA72431) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofAT130-5(AAA72431)at10?g/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2?g/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb01055-23.0*,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.*Ab01055isaduplicateofAAA72431;theysharethesamesequence.)
IF (Immunofluorescence)
(ImmunofluorescencestainingoffixedRAW264.7cellswithanti-CD32antibodyAT130-5(AAA72431) ImmunofluorescenceanalysisofparaformaldehydefixedRAW264.7cellsonShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofAT130-5(AAA72431)at10?g/mlfor1hfollowedbyAlexaFluor488secondaryantibody(2?g/ml),showingmembranestaining.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAb01055-23.0*,DAPI,mergedchannelsandanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.*Ab01055isaduplicateofAAA72431;theysharethesamesequence.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedmousesplenocyteswithanti-CD32antibodyAT130-5(AAA72431) Immunofluorescenceanalysisofparaformaldehydefixedmouse(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofAT130-5(AAA72431)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembranestaininginasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72431,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
IF (Immunofluorescence)
(Immunofluorescencestainingoffixedmousesplenocyteswithanti-CD32antibodyAT130-5(AAA72431) Immunofluorescenceanalysisofparaformaldehydefixedmouse(Musmusculus)splenocytesonShi-fixcoverslips,permeabilizedwith0.15%TritonstainedwiththechimericrabbitversionofAT130-5(AAA72431)at10ug/mlfor1hfollowedbyAlexaFluor 488secondaryantibody(1ug/ml),showingmembranestaininginasubsetofcells.ThenuclearstainisDAPI(blue).Panelsshowfromleft-right,top-bottomAAA72431,DAPI,mergedchannelsandanisotypecontrol.Theisotypecontrolwasstainedwithananti-FluoresceinantibodyfollowedbyAlexaFluor 488secondaryantibody.)
Related Product Information for CD32 recombinant antibody
The original antibody was generated by immunizing BALB/c FcgammaRIIB—/— mice with FcgammaRIIB-rat CD4 domain 3/4 fusion protein.
References
Williams et al. Development and characterization of monoclonal antibodies specific for the murine inhibitory FcgammaRIIB (CD32B). Eur J Immunol. 2012 Aug;42(8):2109-20. doi: 10.1002/eji.201142302. N/A22760702
Note on publication:
The original publication focuses on the development and characterization of monoclonal antibodies specific for murine inhibitory FcgammaRIIB (CD32B).
Note on publication:
The original publication focuses on the development and characterization of monoclonal antibodies specific for murine inhibitory FcgammaRIIB (CD32B).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
– Da
NCBI Official Full Name
Low affinity immunoglobulin gamma Fc region receptor II
NCBI Official Synonym Full Names
Fc receptor, IgG, low affinity IIb
NCBI Official Symbol
Fcgr2b
NCBI Official Synonym Symbols
CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; fcRII; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-b; FcgammaRIIB; Fc-gamma-RII; fc-gamma RII; Fc gamma RIIB; fc-gamma-RIIB; fc gamma receptor IIB; lymphocyte antigen 17; igG Fc receptor II beta
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II
UniProt Gene Name
Fcgr2
UniProt Synonym Gene Names
Fcgr2b; Ly-17; Fc-gamma RII; Fc-gamma-RIIB; FcRII; Ly-17
UniProt Entry Name
FCGR2_MOUSE
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Product Notes
The CD32 fcgr2 (Catalog #AAA72431) is a Recombinant Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD32 [AT130-5], Rat IgG1, kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD32 can be used in a range of immunoassay formats including, but not limited to, Blocking, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD32 fcgr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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