FCM/FACS (Flow Cytometry)
(Separation of human CD56 positive CD3 negative lymphocytes (red-filled) from CD56 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD56 Antibody | anti-CD56 antibody
Anti-Hu CD56 PE-Cy5
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD56; N/A; Anti-Hu CD56 PE-Cy5; neural cell adhesion molecule 1; NCAM1; MSK39; anti-CD56 antibody
Reactivity
Human
Isotype
Mouse IgG2a
Clone Number
LT56
Specificity
The mouse monoclonal antibody LT56 recognizes an extracellular epitope of CD56 (NCAM), a transmembrane glycoprotein expressed ubiquitously in the nervous system and found also on T cells and NK cells.
Form/Format
PE-Cy5
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffe
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 5 (PE-Cy5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffe
Applicable Applications for anti-CD56 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Cell line KG1a
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD56 positive CD3 negative lymphocytes (red-filled) from CD56 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD56 positive CD3 negative lymphocytes (red-filled) from CD56 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining of human lymphocytes stained usinganti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining of human lymphocytes stained usinganti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD56 (LT56) PE-Cy™5 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD56 antibody
CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.
References
*Munteanu AN, Surcel M, Huica RI, Isvoranu G, Constantin C, Pirvu IR, Chifiriuc C, Ulmeanu C, Ursaciuc C, Neagu M: Peripheral immune cell markers in children with recurrent respiratory infections in the absence of primary immunodeficiency. Exp Ther Med 2019, June 26, 1693-1700, URL: https://www.spandidos-publications.com/10.3892/etm.2019.7714,
*McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/17581419?ordin,*Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17583777?ordin,*Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/17900814?ordin,*Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1710251?ordina,
*McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/17581419?ordin,*Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17583777?ordin,*Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/17900814?ordin,*Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1710251?ordina,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
858
NCBI Official Full Name
Neural cell adhesion molecule 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
NCAM1
NCBI Official Synonym Symbols
CD56; NCAM; MSK39
NCBI Protein Information
neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal antibody 5.1H11
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Gene Name
NCAM1
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1
UniProt Entry Name
NCAM1_HUMAN
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Product Notes
The CD56 ncam1 (Catalog #AAA129239) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD56 PE-Cy5 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD56 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD56 ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD56, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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