anti-Human CD9-Cy7 Antibody | anti-CD9 antibody

Anti-Hu CD9 PE-Cy7

Cat. Num. AAA129221

• Gene Names: CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD9-Cy7; N/A; Anti-Hu CD9 PE-Cy7; CD9 molecule; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; BA2; anti-CD9 antibody

• Reactivity: Human
• Isotype: Mouse IgG1
• Clone Number: MEM-61
• Specificity: The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• Form/Format: PE-Cy7; Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffe

• Applicable Applications for anti-CD9 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Pre-B cell line NALM-6.
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD9 positive CD45 negative thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD9 (MEM-61) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD9 (MEM-61) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD9 antibody

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

References

*Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1;167(5):2724-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/11509616?itool=,*Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol. 2007 Nov;293(5):C1660-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17855771?itool=,*Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr;20(7):2030-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/19211836?itool,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL:,
*Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9., URL: https://pubmed.ncbi.nlm.nih.gov/17190803/,*Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96., URL: https://pubmed.ncbi.nlm.nih.gov/17668233/,*Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7., URL: https://pubmed.ncbi.nlm.nih.gov/18000614/,*Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65., URL: https://pubmed.ncbi.nlm.nih.gov/17018612/,

NCBI and Uniprot Product Information

• NCBI GI #: 34769
• NCBI GeneID: 928
• UniProt Accession #: P21926; Q5J7W6; Q96ES4; D3DUQ9
• Molecular Weight: 25,416 Da
• NCBI Official Full Name: MRP-1 (motility related protein)
• NCBI Official Synonym Full Names: CD9 molecule
• NCBI Official Symbol: CD9
• NCBI Official Synonym Symbols: MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
• NCBI Protein Information: CD9 antigen; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein
• UniProt Protein Name: CD9 antigen
• UniProt Gene Name: CD9
• UniProt Synonym Gene Names: MIC3; TSPAN29; MRP-1; Tspan-29
• UniProt Entry Name: CD9_HUMAN