anti-Human CD97 Antibody | anti-CD97 antibody

Anti-Hu CD97 APC

Cat. Num. AAA129220

• Gene Names: CD97; TM7LN1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD97; N/A; Anti-Hu CD97 APC; adhesion G protein-coupled receptor E5; TM7LN1; ADGRE5; anti-CD97 antibody

• Reactivity: Human
• Isotype: Mouse IgG1
• Clone Number: MEM-180
• Specificity: The antibody MEM-180 recognizes a unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD97 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: PHA-activated peripheral blood cells
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD97 (MEM-180) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD97 (MEM-180) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD97 antibody

CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.

References

*Wobus M, Vogel B, Schmücking E, Hamann J, Aust G: N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding. Int J Cancer. 2004 Dec 10;112(5):815-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/15386373,
*Wang T, Tian L, Haino M, Gao JL, Lake R, Ward Y, Wang H, Siebenlist U, Murphy PM, Kelly K: Improved antibacterial host defense and altered peripheral granulocyte homeostasis in mice lacking the adhesion class G protein receptor CD97. Infect Immun. 2007 Mar;75(3):1144-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/17158902?ordin,*Abbott RJ, Spendlove I, Roversi P, Fitzgibbon H, Knott V, Teriete P, McDonnell JM, Handford PA, Lea SM: Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55. J Biol Chem. 2007 Jul 27;282(30):22023-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/17449467?ordin,*Galle J, Sittig D, Hanisch I, Wobus M, Wandel E, Loeffler M, Aust G: Individual cell-based models of tumor-environment interactions: Multiple effects of CD97 on tumor invasion. Am J Pathol. 2006 Nov;169(5):1802-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/17071601?ordin,*Visser L, de Vos AF, Hamann J, Melief MJ, van Meurs M, van Lier RA, Laman JD, Hintzen RQ. Expression of the EGF-TM7 receptor CD97 and its ligand CD55 (DAF) in multiple sclerosis. J Neuroimmunol. 2002 Nov;132(1-2):156-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/12417446?ordin,

NCBI and Uniprot Product Information

• NCBI GI #: 68508955
• NCBI GeneID: 976
• NCBI Accession #: NP_001020331.1
• NCBI GenBank Nucleotide #: NM_001025160.2
• UniProt Accession #: P48960; O00718; O76101; Q8NG72; Q8TBQ7; A8K7Z4; B2RBJ9
• Molecular Weight: 91,869 Da
• NCBI Official Full Name: CD97 antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: CD97 molecule
• NCBI Official Symbol: CD97
• NCBI Official Synonym Symbols: TM7LN1
• NCBI Protein Information: CD97 antigen; leukocyte antigen CD97; seven-span transmembrane protein; seven transmembrane helix receptor; seven-transmembrane, heterodimeric receptor associated with inflammation
• UniProt Protein Name: CD97 antigen
• UniProt Gene Name: CD97
• UniProt Entry Name: CD97_HUMAN