WB (Western Blot)
(Western blot analysis of CD97 expression in HepG2 whole cell lysate; HCT116 whole cell lysate;, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse CD97 Polyclonal Antibody | anti-CD97 antibody
CD97 Antibody
Gene Names
ADGRE5; CD97; TM7LN1
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CD97, Antibody; CD97 Antibody; ADGRE5; Adhesion G protein-coupled receptor E5; CD 97; CD_antigen=CD97; CD97; CD97 antigen; CD97 antigen subunit beta; CD97 molecule; CD97_HUMAN; Leukocyte antigen CD97; Seven span transmembrane protein; Seven transmembrane helix receptor; Seven transmembrane heterodimeric receptor associated with inflammation; TM7LN 1; TM7LN1; anti-CD97 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CD97 antibody detects endogenous levels of total CD97
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
786
Applicable Applications for anti-CD97 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane and Secreted > Extracellular Space.
Tissue Specificity
Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompanied by detectable levels of soluble CD97 in the synovial fluid.
Conjugation
Unconjugated
WB (Western Blot)
(Western blot analysis of CD97 expression in HepG2 whole cell lysate; HCT116 whole cell lysate;, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of CD97 expression in HepG2 whole cell lysate; HCT116 whole cell lysate;, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA323374 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323374 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CD97 antibody
Function: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration (By similarity).
Subunit Structure: Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) interacts with chondroitin sulfate.
Post-translational Modifications: Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
Similarity: The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.Binding to chondroitin sulfate is mediated by the fourth EGF domain. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Subunit Structure: Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) interacts with chondroitin sulfate.
Post-translational Modifications: Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
Similarity: The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.Binding to chondroitin sulfate is mediated by the fourth EGF domain. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 92 kDa
Predicted: 92 kDa
Predicted: 92 kDa
NCBI Official Full Name
CD97 antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor E5
NCBI Official Symbol
ADGRE5
NCBI Official Synonym Symbols
CD97; TM7LN1
NCBI Protein Information
CD97 antigen
UniProt Protein Name
CD97 antigen
UniProt Gene Name
CD97
Similar Products
Product Notes
The CD97 cd97 (Catalog #AAA323374) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD97 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD97 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD97 cd97 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD97, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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