WB (Western Blot)
(Western blot analysis on human serum using anti- Complement C3 mouse monoclonal antibody.)
anti-Human Complement C3 Antibody | anti-C3 antibody
Complement C3 (betachain) Antibody
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human
Applications
Western Blot
Synonyms
Complement C3; N/A; Complement C3 (betachain) Antibody; anti-C3 antibody
Reactivity
Human
Clone Number
11-10
Specificity
This antibody is produced by immunizing mouse with a synthetic peptide (KLH-coupled) corresponding to the residues of Complement C3.
Form/Format
Liquid; 1*TBS (pH7.4), 0.5% BSA, 25% Glycerol.
Preservative: 0.05% Sodium Azide.
Preservative: 0.05% Sodium Azide.
Concentration
0.37mg/ml (varies by lot)
Sequence Length
1663
Applicable Applications for anti-C3 antibody
Western Blot (WB)
Application Notes
WB: 1:2,000
Preservative
0.05% Sodium Azide.
Cellular Localization
Secretory Protein
Positive Control
Human plasma
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis on human serum using anti- Complement C3 mouse monoclonal antibody.)
WB (Western Blot)
(Western blot analysis on human serum using anti- Complement C3 mouse monoclonal antibody.)
Related Product Information for anti-C3 antibody
Human Complement C3 (C3) is synthesized as a single-chain pro-molecule (185 kDa) that tsuffers several posttranslational modifications. Before being a mature protein, C3 is split into beta-chain (70 kDa) and alpha chain (115 kDa) and forms a rare internal thioester bond. C3 plays a central role in the activation of the complement system. It’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
References
"Structure of C3b reveals conformational changes that underlie complement activity."Janssen B.J.C., Christodoulidou A., McCarthy A., Lambris J.D., Gros P.Nature 444:213-216(2006). "Disulfide bridges in human complement component C3b."Dolmer K., Sottrup-Jensen L.FEBS Lett. 315:85-90(1993). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry."Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kDa
NCBI Official Full Name
complement C3 preproprotein
NCBI Official Synonym Full Names
complement C3
NCBI Official Symbol
C3
NCBI Official Synonym Symbols
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
NCBI Protein Information
complement C3
UniProt Protein Name
Complement C3
UniProt Gene Name
C3
UniProt Synonym Gene Names
CPAMD1; C3bc; ASP
Similar Products
Product Notes
The C3 c3 (Catalog #AAA17956) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Complement C3 (betachain) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Complement C3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:2,000. Researchers should empirically determine the suitability of the C3 c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement C3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.