IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Cytokeratin 8 antibody TS1 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TS1 (AAA72375) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72375, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Human Cytokeratin 8 Antibody | anti-KRT8 antibody
Anti-Cytokeratin 8 [TS1]
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
Purity
Purified. Protein A affinity purified
Synonyms
Cytokeratin 8; N/A; Anti-Cytokeratin 8 [TS1]; CK8; CK-8; CK 8; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; TS 1; Mab 202; anti-KRT8 antibody
Reactivity
Human
Isotype
Rabbit IgG kappa
Clone Number
TS1
Specificity
This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340-365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-KRT8 antibody
IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Cytokeratin 8 antibody TS1 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TS1 (AAA72375) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72375, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Cytokeratin 8 antibody TS1 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TS1 (AAA72375) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72375, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western blot using anti-Cytokeratin 8 antibody TS1 . A431 cells lysate (35ug protein in RIPA buffer) was resolved on an SDS-PAGE gel and blots were probed with the chimeric rabbit version of TS1 (AAA72375) at 0.3ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-Cytokeratin 8 antibody TS1 . A431 cells lysate (35ug protein in RIPA buffer) was resolved on an SDS-PAGE gel and blots were probed with the chimeric rabbit version of TS1 (AAA72375) at 0.3ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Cytokeratin 8 antibody TS1 . Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of TS1 (AAA72375, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-Cytokeratin 8 antibody TS1 . Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of TS1 (AAA72375, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-KRT8 antibody
The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.
Describes the generation and characterization of 10 antibodies against cytokeratin which are expressed on normal and malignant epithelial cells.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Describes the generation and characterization of 10 antibodies against cytokeratin which are expressed on normal and malignant epithelial cells.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Product Categories/Family for anti-KRT8 antibody
References
Sundström et al.
J Histochem Cytochem. 1989 Dec;37(12):1845-54.
J Histochem Cytochem. 1989 Dec;37(12):1845-54.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,704 Da
NCBI Official Full Name
keratin, type II cytoskeletal 8 isoform 2
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
NCBI Protein Information
keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8; CK-8; K8
UniProt Entry Name
K2C8_HUMAN
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Product Notes
The KRT8 krt8 (Catalog #AAA72375) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 8 [TS1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 8 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), ELISA. Researchers should empirically determine the suitability of the KRT8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 8, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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