Bioactivity
CD28/TP44 active protein
Recombinant Human/Cynomolgus CD28/TP44 (N-His)
Gene Names
CD28; Tp44
Purity
>95% as determined by reducing SDS-PAGE
Synonyms
CD28/TP44; N/A; Recombinant Human/Cynomolgus CD28/TP44 (N-His); CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44; CD28/TP44 active protein
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE
Form/Format
Lyophilized from a 0.2um filtered solution of 20mM Tris-HCl, 150mM NaCl, pH8.0.
Sequence
Asn19-Pro152
Species
Human, Cynomolgus
Tag
N-6His
Endotoxin
<1 IEU/ug as determined by LAL test.
Protein Construction
Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.
Activity
Immobilized Human/Cynomolgus CD28-His at 2ug/ml (100ul/well) can bind Anti-CD28 mAb . The ED50 of Anti-CD28 mAb is 15.3ng/ml.
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
Bioactivity
Bioactivity
SDS-PAGE
SDS-PAGE
Related Product Information for CD28/TP44 active protein
T-cell-specific surface glycoprotein CD28(CD28) is a single-pass typeI membrane protein which contains one Ig-likeV-type (immunoglobulin-like) domain. It belongs to the immunoglobulin(Ig) superfamily. CD28 is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation.CD28 co-stimulation is necessary for CD4 positive T-cell proliferation and survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Product Categories/Family for CD28/TP44 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,066 Da
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28 isoform 2
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
CD28
NCBI Official Synonym Symbols
Tp44
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; CD28 antigen
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Gene Name
CD28
UniProt Entry Name
CD28_HUMAN
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Product Notes
The CD28/TP44 cd28 (Catalog #AAA177933) is an Active Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Asn19-Pro1 52. It is sometimes possible for the material contained within the vial of "CD28/TP44, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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